- Product Name
- Rat IL-1β (Interleukin 1 Beta) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in plasma, tissue homogenates and other biological fluids.
- < 18.75pg/ml
- UniProt ID
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 87-105 94 EDTA plasma(n=5) 86-105 95 heparin plasma(n=5) 92-104 97
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 90-104% 94-104% 95-102% 90-105% EDTA plasma(n=5) 82-94% 91-98% 86-101% 85-100% heparin plasma(n=5) 80-92% 80-95% 88-100% 80-98%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area