Products

Rat IL-1β (Interleukin 1 Beta) ELISA Kit

ER1094 Catalogue No.
Reactivity
Rat
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Rat IL-1β (Interleukin 1 Beta) ELISA Kit
Catalogue No.
ER1094
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Q63264
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10594
EDTA plasma(n=5)86-10595
heparin plasma(n=5)92-10497
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-104%94-104%95-102%90-105%
EDTA plasma(n=5)82-94%91-98%86-101%85-100%
heparin plasma(n=5)80-92%80-95%88-100%80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only