Products

Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

EM0183 Catalogue No
TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Reactivity
Mouse
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No.
EM0183
Alias
TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNFα ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Mouse
UniProt ID
P06804
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10396
EDTA plasma(n=5)86-9891
heparin plasma(n=5)91-10297
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)92-96%88-101%87-103%88-102%
EDTA plasma(n=5)82-101%82-98%82-93%82-99%
heparin plasma(n=5)83-97%90-100%89-98%89-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only