FineTest® protein expression platform solves various difficulties in protein expression and expression to meet different applications. Our powerful R & D team is committed to be your best experimental partner and offers customers well-designed protein sequences, the best vector and label, greatly ensuring solubility and activity of proteins.
FineTest® protein services provided over 5,000 recombinant proteins expressed by HEK293 and E.Coli for WB, ELISA application, covering common species(e.g. human) for inflammatory factors, signaling pathways, diagnostic materials, receptor proteins, enzymes, antigens etc. Besides His tag and GST tag, we developed special IF2DI tag with high-efficient solubility which can greatly improve the protein expression. Featured services like protein labeling and conjugation are also available. Also we accept custom protein production.
FineTest® protein expression platform uses multiple purification methods to maintain the protein purity, including affinity purification, ion exchange, hydrophobic chromatography etc. We also have advanced refolding technology for insoluble proteins, and try our best to restore protein conformation as well as keeping its property to natural protein.
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More Info for Your Viewing Protein Product
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Soluble Protein
The self-developed pET28a-IF2DI expression vector has the powerful IF2DI tag, which has better soluble expression effect on very hydrophobic proteins. Perfectly replace tags like GST, MBP etc. The smaller molecular weight has a lower effect on the expression of target proteins.
Unlabeled Protein
According to customers' requirements, IF2DI tag can be removed by Thrombin cleavage site to obtain the unlabeled protein. There is no effect on the protein activity.
Inclusion Body Protein
For insoluble protein aggregates, FineTest uses the unique protein refolding technolog to refold in the suitable buffer system and also obtain the target protein in the form of supernatant. The activity of nearly 100 proteins has been validated.
Activity Validation
Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.The ED50 for this effect is 60-230 ng/mL in a fluorometric assay.
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Activity: Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-8 ng/mL.
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Prokaryotic Expression System
Over 5,000 proteins expressed by E.Coli; High Purity, Better Activity, Widely Applications.
Eukaryotic Expression System
Active proteins expressed by mammalian cells like HEK293, CHO etc.
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High Purity: Over 95%, Validated by SDS PAGE; |
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High Activity: Validated Catalytic and Cellular Activity; |
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Low Endotoxin Level: less than 1EU/μg; |
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Various Species: Human, Rat, Mouse, Monkey; |
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Self-developed, animal free; |
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His tag, Fc tag and unlabeled proteins offered |
| ELISA Comparison between FineTest® P2750 Recombinant CD147 and R&D 972-EMN Recombinant CD147 | ||
|---|---|---|
| Conc. (pg/ml) |
OD(450nm) | |
| R&D | FineTest® | |
| 7.8125 | 0.079 | 0.090 |
| 15.625 | 0.308 | 0.379 |
| 31.25 | 0.532 | 0.624 |
| 62.5 | 0.858 | 0.960 |
| 125 | 1.377 | 1.555 |
| 250 | 2.071 | 2.297 |
| 500 | 2.853 | 2.961 |
| 1000 | 3.383 | 3.322 |
Custom Recombinant Protein
FineTest can produce any custom protein according to customers' requirements. Only protein name, Uniprot ID and applications are required.
| Service Items | Service Checklist | Service Timeline |
| Gene Synthesis | Construction of Expression Plasmid | 1-2 weeks |
| Protein Expression | Transformation Competent Cells | Within 1 week |
| Protein Purification | Column Chromatography Purification or Refolding | 1 week |
| Project Delivery | Refolding, Lyophilized Protein, SDS-PAGE Result | 2-3 weeks |
Since 2016, FineTest products have been used by global and domestic scientific research institutions, universities and pharmaceutical platforms. The quantity of citations are always rapidly increasing due to the investment on R & D of our products. Currently, over 3,300 citations have proven the stable quality widely accepted by our customers. The total IF is nearly 12,000. The highest and average IF are 46.351 and 3.51 respectively. There is a fast annual increase of citations and IF. Especially some of journals are highly ranked in the discipline.(e.g. Cell Research, Cell, Advanced Materials, Annals of the Rheumatic Diseases, Materials Today, Cellular & Molecular Immunology, Journal of Medical Virology etc.)
Theranostics IF: 11.556
- Cat.No
- Product Name
- P5500
- Recombinant Human TMSB4X
International Journal of Biological Sciences IF: 10.75
- Cat.No
- Product Name
- P0633
- Recombinant Human gpx4
Acta Pharmaceutica Sinica B IF: 14.907
- Cat.No
- Product Name
- P8489
- Recombinant Human HIF1A
Cellular & Molecular Immunology IF: 24.1
- Cat.No
- Product Name
- P1226
- Recombinant Human SIRT2
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