Flow Cytometry(FCM) is the rapid qualitative and quantitative analysis for multi-parameter and sorting technology, using flow cytometer to measure cells or particles irradiated by particular wavelength laser in a fast and straight-line flow state. Detected samples include various cells(e.g. peripheral blood, bone marrow, suspension or adhension cultured cells), supernatant(plasma, serum, cell culture fluid, lysate, biopsy puncture fluid), microorganisms and synthetic microspheres etc.
Applications
Widely used in the fields of immunology, medicine and biology etc, such as cell phenotype identification, cell sorting, cytokine measurement, cell proliferation, cell apoptosis, cell cycle, cell killing etc.
Comparison between Flow Cytometry and ELISA
| Assay | Antibody | Proper Device | Marker | Reactant | Reaction Type | Measurement Indicator | Result Reading | Protein Expression |
|---|---|---|---|---|---|---|---|---|
| Flow Cytometry | flow cytometry antibody | flow cytometer | fluorescent substances | fluorescent group | fluorescence excitation | emission | fluorescence value | Intracellular, cell surface, sorting |
| ELISA | ELISA Antibody Pair | microplate reader | biotin-streptavidin-HRP | TMB | enzyme-catalyzed reaction | absorbance | OD value | extracellular |
Flow cytometry antibodies are particularly applied in flow cytometry, accurately measuring and quantitatively analyzing for specific proteins on the cell surface or in the cell. Flow cytometry antibodies are widely applied in the fields of biomedical research, clinical diagnosis, drug development and bioengineering etc.
FineTest developed classically cloned and purified antibody with high affinity and specificity based on the monoclonal antibody development platform, covering human, rat and mouse etc. Various targets are available for your choice. Meanwhile, FineTest offers integrative flow cytometry solution including free colour matching, assay guidance and data analysis services etc.
Hot Selling CD Markers
Flow Cytometry Colour Matching for Immunocyte Subpopulations
| Species | Cell Populations | Flow Cytometry Antibody Combination | Cat.No |
| Human | T/B/NK cell populations detection | CD45-PerCP | PCP-30039 |
| CD3-FITC | FITC-30004 | ||
| CD16-PE | PE-30061 | ||
| CD56-PE | PE-30008 | ||
| CD19-APC | APC-30066 | ||
| Human | Thl/Th2 cell populations detection | CD3-PerCP/Cyanine5.5 | PCP55-30004 |
| CD4-FITC | FITC-30005 | ||
| IFN-γ-PE | PE-30053 | ||
| IL4-APC | APC-30043 | ||
| Mouse | Thl/Th2 cell populations detection | CD3-PerCP/Cyanine5.5 | PCP55-30002 |
| CD4-FITC | FITC-30001 | ||
| IFN-γ-PE | PE-30074 | ||
| IL4-APC | APC-30026 | ||
| Human | Treg cell populations detection | CD4-FITC | FITC-30005 |
| CD25-PE | PE-30035 | ||
| CD127-APC | APC-30033 | ||
| Mouse | Treg cell populations detection | CD4-FITC | FITC-30001 |
| CD25-PE | PE-30017 | ||
| FOXP3-APC | APC-30055 |
Isotype
Isotype uses immunoglobulin and its subtype to match the primary antibody in the same species, Ig subclass and volume, removing background fluorescence caused by non-specific binding between the antibody and the cell.
A List of Isotype Control Antibodies