- Product Name
- Rat INS(Insulin) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- INS ELISA Kit allows for the in vitro quantitative determination of INS concentrations in plasma, tissue homogenates and other biological fluids.
- < 46.875pg/ml
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of INS and the recovery rates were calculated by comparing the measured value to the expected amount of INS in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-100 93 EDTA plasma(n=5) 87-94 91 heparin plasma(n=5) 93-104 98
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 95-103% 85-101% 92-104% 85-103% EDTA plasma(n=5) 82-99% 91-100% 82-100% 82-101% heparin plasma(n=5) 85-97% 84-98% 82-95% 81-97%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area