FineTest® adopts a new polymerase-mark system(including Poly-HRP Goat Anti-Rabbit IgG and Poly-HRP Goat Anti-Mouse IgG). More enzymes will bind to the single antibody through the arm structure. Meanwhile, the optimal structure of current system enhances the signal during immunohistochemistry and reduces non-specific reaction as well as simplifies experimental steps.
Comparison between Different Detection Systems
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Amplification System |
Size |
Components |
The Experimental Steps |
Signal |
|
PAP, APAAP |
Very large＞5000kd |
Anti-HRP-IgG HRP HRP-IgG |
|
Medium |
|
ABC(SABC) |
Large＞2000kd |
Avidin(Streptavidin) Biotin-HRP |
|
Strong |
|
Poly-HRP-IgG |
Medium:700~1200kd |
HRP(multiple)-IgG (secondary antibody) |
1. primary antibody 2. secondary antibody |
Very strong |
|
HRP-IgG(normal) |
Small:200~282kd |
HRP(1-3)-IgG (secondary antibody) |
1. primary antibody 2. secondary antibody |
Weak or none |
Staining Comparison between Different Vendors
DAB staining for 1min.
SABC staining for 1min.
DAB staining for 1min.
DAB staining for 1min.
Schematic Diagram
IHC Detection Results
IHC assay for target antigens with high and low concentration. Primary and Poly-HRP secondary antibodies as well as DAB kits are offered by FineTest.
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Higher concentration of target antigen |
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Lower concentration of target antigen |
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Antibodies |
Dilution Ratio |
Location |
Cat. No. |
|
Antibodies |
Dilution Ratio |
Location |
Cat. No. |
|
Histone H3.3(A) |
1:150 |
Nucleus |
|
TRX- 1(H) |
1:300 |
Cytoplasm, Nucleus |
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|
PCNA(B) |
1:200 |
Nucleus |
|
PXMP2(I) |
1:150 |
Peroxisome membrane |
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a-Tubulin(C) |
1:300 |
Cytoplasm, Cytoskeleton |
|
TTK(J) |
1:200 |
Cytoskeleton |
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GAPDH(D) |
1:500 |
Cytoplasm, Nucleus |
|
MEK3(K) |
1:300 |
Cytoplasm, Nucleus |
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|
Lamin A/C(E) |
1:400 |
Nucleus |
|
MAP3K3(L) |
1:300 |
Cytoplasm |
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VDAC1(F) |
1:400 |
Cell membrane, tochondrion |
|
HDAC5(M) |
1:250 |
Cytoplasm, Nucleus |
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|
β-actin(monoclonal)(G) |
1:250 |
Cytoplasm, Cytoskeleton |
|
P2RX7(N) |
1:250 |
Cell membrane |
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Validated Images
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Negative Control
Human heart tissue(1), human colon cancer tissue(2), rat iver tissue(3). human breast cancer tissue(4). Primary antibody is negative serum, Poly-HRP Goat anti rabbit IgG and Poly-HRP Goat anti mouse IgG are diluted at ratio of 1:50. DAB staining for 10min. Nucleus is counterstained by hematoxylin.
Product List
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Catalogue No. |
Product Name |
Tested Application |
|
Poly HRP-conjugated Affinipure Goat Anti-Mouse/Rabbit IgG (H+L) |
IHC, ELISA |
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Poly HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) |
ELISA, WB, IHC |
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Poly HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) |
ELISA, WB, IHC |
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SABC one-component |
ELISA |
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SABC two-component |
IHC |
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Rabbit-DAB (Poly-HRP) Detection IHC Kit |
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Mouse-DAB (Poly-HRP) Detection IHC Kit |
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