Since 2014, FineTest begins to produce ELISA kits with a well-established R & D system for ELISA kits, antibodies and proteins. Based on known gene sequence of monkeypox virus(MPXV), FineTest has successfully developed various MPXV - related proteins expressed by E.Coli and ELISA kits.
Monkeypox virus(MPXV) is an enveloped double-stranded DNA virus, and belongs to orthopoxvirus genus in the poxvirudae family. Similar to viruses in the same genus, MPXV is likely to replicate uniquely morphological intracellular mature virus(IMV) and extracellular enveloped virus(EEV) in the host's cytoplasm. IMV has solid physical structure facilitating the transmission among hosts. Weaker EEV is coated by envelope to limit immune clearance, which is suitable for intercellular transmission.
In 1970, isolated human MPXV cases were first found in several African countries. During the past 20 years, the virus was widely distributed in African continent. Since 2022 May, MPXV cases across the world have greatly increased. WHO has to announce Monkeypox epidemic is the global health emergency.
MPXV Pathogenesis
MPXV first enters into human body via respiratory tract or skin, aiming to infect airway epithelial cell or keratinocyte. Then, the virus infects antigen presenting cells(e.g. dendritic cells and macrophages). These cells spread the virus to nearby lymph nodes, causing lymphadenectasis. Then, the virus spreads to large organs(e.g. spleen, liver etc.), skins and gonad via lymphatic system and blood respectively. Finally, the virus forms pustule and ulcer on the skin and mucosa, releasing a large number of virus possibly transmitted via aerosol.
Immunotherapy and prevention strategy are public health tools capable of effectively control the MPXV epidemic with strict contact tracing. Besides, serological diagnostic tools are also very important for knowing the contact history.
Monkeypox Virus Products
FineTest MPXV products are expressed by E.coli system following known gene sequences, and widely applied in immunological research and functional validation. Related ELISA kits, antibodies and proteins are listed below.
Recombinant Proteins
| Cat.No | Product Name | Host | Expression Region | Tag |
|---|---|---|---|---|
| P3262 | Recombinant A29L | E.Coli | 110aa | N-terminal His Tag |
| P3271 | Recombinant Monkeypox Virus A33R | E.Coli | 142aa | N-terminal His Tag |
| P9408 | Recombinant A33R | E.Coli | 1-142 | N-terminal His Tag |
| P3268 | Recombinant A35R | E.Coli | 125aa | N-terminal His Tag |
| P3269 | Recombinant E8L | E.Coli | 275aa | N-terminal His Tag |
| P3263 | Recombinant L1R | E.Coli | 152aa | N-terminal His Tag |
| P3267 | Recombinant M1R | E.Coli | 250aa | N-terminal His Tag |
Antibodies
| Cat.No | Product Name | Host | Clonality | Tested Application |
|---|---|---|---|---|
| FNab10955 | A29L antibody | Rabbit | polyclonal | ELISA, WB |
| FNab10972 | M1R antibody | Rabbit | polyclonal | ELISA, WB |
| FNab10971 | A35R antibody | Rabbit | polyclonal | ELISA, WB |
| FNab10970 | E8L antibody | Rabbit | polyclonal | ELISA, WB |
| FNab10700 | L1R antibody | Rabbit | polyclonal | ELISA, WB |
ELISA Kits
| Cat.No | Product Name | Sensitivity | Range | Detection Method |
|---|---|---|---|---|
| EH5237 | Human Anti-MPV IgM (Anti-Monkeypox Virus IgM) ELISA Kit | qualitative | qualitative | Capture ELISA |
| EH5236 | Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit | qualitative | qualitative | Indirect ELISA |
| EH4996 | Human Anti-MPV IgG(Anti-Monkeypox Virus IgG) ELISA Kit | 1.875ng/ml | 3.125-200ng/ml | Indirect ELISA |
| EH4997 | Human Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit | 0.938ng/ml | 1.563-100ng/ml | Indirect ELISA |
| EM2107 | Mouse Anti-MPV IgG (Anti-Monkeypox Virus IgG)ELISA Kit | 1.875ng/ml | 3.125-200ng/ml | Indirect ELISA |
| EM2128 | Mouse Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit | 0.938ng/ml | 1.563-100ng/ml | Indirect ELISA |
| EP0355 | Porcine Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit | 1.875ng/ml | 3.125-200ng/ml | HRP Indirect ELSIA |