Isotype Control Antibodies

Isotype control uses immunoglobulin and its subtype to match the primary antibody in the same species, Ig subclass and volume, removing background signal caused by non-specific antibody binding.

How to Choose Isotype Control

1. Use unlabelled primary antibody and labelled secondary antibody: the species and Ig subclass of isotype control antibody should be the same as primary antibody. When using labelled primary antibody, isotype control should have the same marker as primary antibody. Use the same testing concentration of isotype control as well to get the correct result analysis.

2. If suitable isotype control is not found, selection priority should be different subchain → different subclass → same category. (following the same species, fluorescence labelling and Ig subclass)
E.g. For a Mouse IgG2а,κ antibody, if the same isotype control is not found in the list, then choose the same fluorescence-labelled Mouse IgG2а → Mouse IgG2b → Mouse IgG2.

Applications

1. Flow Cytometry
Staining Method: Sample Tube: Primary Antibody + Sample; Isotype Control Tube: Isotype Control + Sample; Experimenters should use isotype control to keep the accuracy of flow cytometry.

Cat.No	Product Name	Host	Marker	Size
FITC-30073	FITC Rat IgG2a,κ Isotype Control(2A3)	Rat	FITC	20T/100T
PE-30073	PE Rat IgG2a,κ Isotype Control(2A3)	Rat	PE	20T/100T
APC-30073	APC Rat IgG2a,κ Isotype Control(2A3)	Rat	APC	20T/100T
FITC-30075	FITC Rat IgG1,κ Isotype Control(HRPN)	Rat	FITC	20T/100T
PE-30075	PE Rat IgG1,κ Isotype Control(HRPN)	Rat	PE	20T/100T
APC-30075	APC Rat IgG1,κ Isotype Control(HRPN)	Rat	APC	20T/100T
FITC-30076	FITC Mouse IgG1,κ Isotype Control(MOPC-21)	Mouse	FITC	20T/100T
PE-30076	PE Mouse IgG1,κ Isotype Control(MOPC-21)	Mouse	PE	20T/100T
APC-30076	APC Mouse IgG1,κ Isotype Control(MOPC-21)	Mouse	APC	20T/100T
PCP-30076	PerCP Mouse IgG1,κ Isotype Control(MOPC-21)	Mouse	PerCP	20T/100T
FITC-30077	FITC Mouse IgG2a,κ Isotype Control(C1.18.4)	Mouse	FITC	20T/100T
PE-30077	PE Mouse IgG2a,κ Isotype Control(C1.18.4)	Mouse	PE	20T/100T
APC-30077	APC Mouse IgG2a,κ Isotype Control(C1.18.4)	Mouse	APC	20T/100T
FITC-30078	FITC Mouse IgG2b,κ Isotype Control(MPC-11)	Mouse	FITC	20T/100T
PE-30078	PE Mouse IgG2b,κ Isotype Control(MPC-11)	Mouse	PE	20T/100T
APC-30078	APC Mouse IgG2b,κ Isotype Control(MPC-11)	Mouse	APC	20T/100T

2. Immunohistochemistry and Immunofluorescence

When the staining signal of isotype control is weaker than the specific antibody, the antibody binds with the target protein in the sample.

3. Immunoprecipitation

The figure below shows control IgG. Isotype control sample can only detect the heavy chain band without target protein band.

4. ELISA

When ELISA assay detects a specific antibody, isotype control can be used to eliminate non-specific binding.

Cat.No	Product Name	Host	Size
FNSA-0106	Normal Rabbit IgG Control	Rabbit	1ml
FNSA-0109	Normal Human IgG Control	Human	1ml
FNSA-0130	Normal mouse IgG Control	Mouse	1ml
FNSA-0131	Chicken IgY-Isotype Control	Chicken	1ml
FNSA-0133	Goat IgG-Isotype Control	Goat	10mg
FNSA-0134	Rat IgG-Isotype Control	Rat	10mg

Citations