Products

Rat IL-6(Interleukin-6) ELISA Kit

ER0042 Catalogue No
Reactivity
Rat
Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Product Name
Rat IL-6(Interleukin-6) ELISA Kit
Catalogue No.
ER0042
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Rat
UniProt ID
P20607
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)91-9795
EDTA plasma(n=5)85-10595
heparin plasma(n=5)89-10395
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-104%85-105%92-103%90-105%
EDTA plasma(n=5)82-100%83-99%83-100%83-99%
heparin plasma(n=5)80-100%84-96%82-96%85-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only