Products
Rat IL-6(Interleukin-6) ELISA Kit
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- ER0042
- Alias:
- Interleukin-6 ELISA Kit, IL-6 ELISA Kit, B-cell stimulatory factor 2 ELISA Kit, BSF-2 ELISA Kit, CTL differentiation factor ELISA Kit, CDF ELISA Kit, Hybridoma growth factor ELISA Kit, Interferon beta-2 ELISA Kit, IFN-beta-2 ELISA Kit, IL6 ELISA Kit, IFNB2 ELISA Kit
- Species:
- Rat
- Range:
- 62.5-4000pg/ml
- Sensitivity:
- 37.5pg/ml
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- Product Name
- Rat IL-6(Interleukin-6) ELISA Kit
- Alias
- Interleukin-6 ELISA Kit, IL-6 ELISA Kit, B-cell stimulatory factor 2 ELISA Kit, BSF-2 ELISA Kit, CTL differentiation factor ELISA Kit, CDF ELISA Kit, Hybridoma growth factor ELISA Kit, Interferon beta-2 ELISA Kit, IFN-beta-2 ELISA Kit, IL6 ELISA Kit, IFNB2 ELISA Kit
- Catalogue No.
- ER0042
- Size
- 48T/96T
- Species
- Rat
- UniProt ID
- P20607
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 62.5-4000pg/ml
- Sensitivity
- 37.5pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with IL-6 , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
- Washing: Wash the plate twice without immersing.
- Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
- Washing: Wash the plate three times and immerse for 1min each time.
- Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.129 0.136 0.132 62.5 0.168 0.176 0.171 125 0.179 0.188 0.183 250 0.218 0.229 0.222 500 0.319 0.335 0.325 1000 0.61 0.641 0.622 2000 1.157 1.216 1.181 4000 2.011 2.114 2.052 - Recovery
-
Add a certain amount of IL-6 into the sample. Calculate the recovery by comparing the measured value with the expected amount of IL-6 in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 87-105 98 EDTA plasma(n=10) 85-103 96 Heparin plasma(n=10) 94-105 100 - Linearity
-
Dilute the sample with a certain amount of IL-6 at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 86-103% 86-99% 86-98% EDTA plasma(n=10) 92-98% 90-101% 83-100% Heparin plasma(n=10) 81-100% 80-99% 86-98% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 121.8 501 1984 124.9 497.1 1995 Standard deviation 6.15 25.95 103.76 6.57 23.61 105.54 CV(%) 5.05 5.18 5.23 5.26 4.75 5.29 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
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- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat Gsr(Glutathione reductase) ELISA Kit
- Rat PTGS2/COX-2(Prostaglandin G/H synthase 2) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- AIP Conference Proceedings
- Cited Date:
- 2020-09-16
- Product:
- Journal:
- research square
- Cited Date:
- 2020-09-29
- Product:
-
- Rat PGE2(Prostaglandin E2) ELISA Kit
- Rat 8-iso-PGF2α(8-isoprostane) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
- Rat IL-10(Interleukin-10) ELISA Kit
- Rat IL-13(Interleukin-13) ELISA Kit
- Rat IL-17(Interleukin-17) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Mouse Ltb4r(Leukotriene B4 receptor 1) ELISA Kit
- Journal:
- Research Square
- Author:
- Islamic Azad University
- Cited Date:
- 2024-02-18
- Product:
- Journal:
- Journal of Innovative Research in Life Sciences
- Author:
- Department of Biochemistry, Federal University Gusau, Zamfara State-Nigeria
- Sample:
- serum
- Cited Date:
- 2024-05-31
- Product:
- Journal:
- Recent Advances in Drug Delivery and Formulation
- Author:
- Department of Pharmacy, I.T.S. College of Pharmacy, Delhi-Meerut Road, Ghaziabad -201206, India.
- Cited Date:
- 2024-10-08
- Product:
- Journal:
- Farabi Medical Journal
- Cited Date:
- 2023-04-07
- Product:
- Journal:
- Medicinski Glasnik
- Cited Date:
- 2021-07-15
- Product:
- Journal:
- Sahel Journal of Life Sciences FUDMA
- Author:
- Department of Biochemistry and Molecular Biology, Federal University Dutsin-Ma, P.M.B 5001, Katsina State, Nigeria
- Sample:
- serum
- Cited Date:
- 2024-03-08
- Product:
- Journal:
- Journal of Nutrition and Health
- Cited Date:
- 2020-03-13
- Product:
- Journal:
- Comparative Clinical Pathology
- Cited Date:
- 2019-02-15
- Product:
-
- 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- MDA(Malondialdehyde) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Gpx1(Glutathione peroxidase 1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- F?rat University Medical Journal of Health Sciences
- Author:
- Gaziantep University, Faculty of Medicine, Department of Physiology, Gaziantep, T?RK?YE
- Cited Date:
- 2023-07-07
- Product:
- Journal:
- Phytomedicine Plus
- Author:
- Dairy Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
- Species:
- blood samples
- Cited Date:
- 2024-06-14
- Product:
- Journal:
- F1000Research
- Cited Date:
- 2022-12-16
- Product:
- Journal:
- bioRxiv
- Author:
- Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi, Nigeria
- Cited Date:
- 2023-09-15
- Product:
- Journal:
- Journal of International Dental and Medical Research
- Cited Date:
- 2020-05-02
- Product:
- Journal:
- Research Square
- Cited Date:
- 2022-03-10
- Product:
- Journal:
- HEPATITIS MONTHLY
- Cited Date:
- 2020-05-10
- Product:
-
- Rat MDA(Malonaldehyde) ELISA Kit
- Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat CASP9(Caspase 9) ELISA Kit
- Rat BCL-2(B-Cell Leukemia/Lymphoma 2) ELISA Kit
- Rat Bax(Apoptosis regulator BAX) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Gpx1(Glutathione peroxidase 1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- Beni-Suef University Journal of Basic and Applied Sciences
- Cited Date:
- 2023-01-06
- Product:
-
- 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- Rat ATF6(Activating transcription factor 6) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
- Rat Ddit3(DNA damage-inducible transcript 3 protein) ELISA Kit
- Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Hmgb1(High mobility group protein B1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat MPO(Myeloperoxidase) ELISA Kit
- Rat Casp3(Caspase 3) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit