- Product Name
- Rat IL-6(Interleukin-6) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.
- < 37.5pg/ml
- UniProt ID
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-100 96 EDTA plasma(n=5) 87-103 95 heparin plasma(n=5) 90-103 98
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-101% 85-96% 88-99% 86-100% EDTA plasma(n=5) 83-95% 82-93% 91-96% 86-96% heparin plasma(n=5) 80-100% 84-100% 89-93% 81-97%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area