Traditional sandwich ELISA assay usually takes 3-4 hours. Most detection reagents in the market are concentrated and can't be distinguished from the colour. Dilution is required before use. FineTest developed QuickTest ELISA kits to decrease customers’ operational errors, improve user's experience and get accurate assay result more efficiently. Compared with regular ELISA kits, FineTest QuickTest ELISA kits have more advantages in the same linearity, stability, recovery and storage conditions.
| Category | Regular ELISA Kit | 90min Rapid Assay | 120min Rapid Assay | Advantage Analysis |
|---|---|---|---|---|
| Reaction Duration | 4h | 90min | 120min | a great decrease of assay time |
| Washing Times | 10 | 5 | 7 | Less washing, easy operation |
| Washing Immersion | Y | N | N | Faster washing without waiting |
| Dilution of Detection Reagents | Concentrated, Dilution Required | Ready-to-Use without dilution | Ready-to-Use without dilution | Avoid improper reagent preparation |
| Distinguished Reagent Colour | N | coloured | coloured | Avoid missing and mistaking in sample loading, improve loading efficiency |
| Precision | CV<8% | CV<6% | CV<6% | Lower CV, Higher Precision |
| Numbers of Reagents | 9 | 6 | 7 | Less Reagents |
Components Comparison between Common and QuickTest ELISA Kits
| No. | Item | Size(48T) | Size(96T) |
|---|---|---|---|
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 |
| E002 | Lyophilized Standard | 1vial | 2vial |
| E003 | Biotin-labeled Antibody(Concentrated, 100X) | 60ul | 120ul |
| E034 | HRP-Streptavidin Conjugate(SABC, 100X) | 60ul | 120ul |
| E024 | TMB Substrate | 5ml | 10ml |
| E039 | Sample Dilution Buffer | 10ml | 20ml |
| E040 | Antibody Dilution Buffer | 5ml | 10ml |
| E049 | SABC Dilution Buffer | 5ml | 10ml |
| E026 | Stop Solution | 5ml | 10ml |
| E038 | Wash Buffer(25X) | 15ml | 30ml |
| E006 | Plate Sealer | 3 pieces | 5 pieces |
| E007 | Product Description | 1 copy | 1 copy |
| No. | Item | Size(48T) | Size(96T) |
|---|---|---|---|
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 |
| E002 | Lyophilized Standard | 1vial | 2vial |
| E054 | Cap/Det Ab | 3ml | 6ml |
| E053 | HRP-Streptavidin(orange) | 5ml | 10ml |
| E024 | TMB Substrate | 5ml | 10ml |
| E039 | Sample Dilution Buffer(blue) | 20ml | 20ml |
| E026 | Stop Solution | 5ml | 5ml |
| E038 | Wash Buffer(25X) | 15ml | 30ml |
| E006 | Plate Sealer | 3 pieces | 5 pieces |
| E007 | Product Description | 1 copy | 1 copy |
| No. | Item | Size(48T) | Size(96T) |
|---|---|---|---|
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 |
| E002 | Lyophilized Standard | 1vial | 2vial |
| E055 | Cap/Det Ab | 3ml | 6ml |
| E024 | TMB Substrate | 5ml | 10ml |
| E039 | Sample Dilution Buffer (blue) | 20ml | 20ml |
| E026 | Stop Solution | 5ml | 5ml |
| E038 | Wash Buffer(25X) | 15ml | 30ml |
| E006 | Plate Sealer | 3 pieces | 5 pieces |
| E007 | Product Description | 1 copy | 1 copy |
Assay Principle and Procedure
antibody added and Standard/sample, color: blue
HRP-Streptavidin added, color: orange
TMB Substrate added, colorless to blue
Stop Solution added, color: blue to yellow
1. 90min Rapid Assay(Double antibody-Sandwich ELISA)
1.1. Assay Flowchart
1.2. Assay Principle
Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.
1.3. Assay Procedure Summary
Step 1: Take out the required strips, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37°C.
Washing: Wash the plate five times without immersion.
Step 2: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C(Accurate TMB visualization control is required.).
Step 3: Add 50ul stop solution. Read at 450nm immediately and calculate.
1.4. Kit Components and Storage
| No. | Item | Size(48T) | Size(96T) | Storage Condition for Opened Kit |
|---|---|---|---|---|
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E002 | Lyophilized Standard | 1vial | 2vial | Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E055 | Cap/Det Ab (Ready to use) | 3ml | 6ml | 2-8°C (Avoid Direct Light) |
| E024 | TMB Substrate | 5ml | 10ml | |
| E039 | Sample Dilution Buffer (blue) | 20ml | 20ml | 2-8°C |
| E026 | Stop Solution | 5ml | 5ml | |
| E038 | Wash Buffer(25X) | 15ml | 30ml | |
| E006 | Plate Sealer | 3 pieces | 5 pieces | |
| E007 | Product Description | 1 copy | 1 copy |
2. 120min Rapid Assay(Double antibody-Sandwich ELISA)
2.1. Assay Flowchart
2.2. Assay Principle
Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, HRP-Streptavidin was added to incubate. Repeat the washing process and then TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.
2.3. Assay Procedure Summary
Step 1: Take out the required strips, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37°C.
Washing: Wash the plate twice without immersion.
Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37°C.
Washing: Wash the plate five times without immersion.
Step 3: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C(Accurate TMB visualization control is required.).
Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.
2.4. Kit Components and Storage
| No. | Item | Size(48T) | Size(96T) | Storage Condition for Opened Kit |
|---|---|---|---|---|
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E002 | Lyophilized Standard | 1vial | 2vial | Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E054 | Cap/Det Ab(Ready to use) | 3ml | 6ml | 2-8°C (Avoid Direct Light) |
| E053 | HRP-Streptavidin(Ready to use, orange) | 5ml | 10ml | |
| E024 | TMB Substrate | 5ml | 10ml | |
| E039 | Sample Dilution Buffer(blue) | 20ml | 20ml | 2-8°C |
| E026 | Stop Solution | 5ml | 5ml | |
| E038 | Wash Buffer(25X) | 15ml | 30ml | |
| E006 | Plate Sealer | 3 pieces | 5 pieces | |
| E007 | Product Description | 1 copy | 1 copy |