Products

Rat E2(Estradiol) ELISA Kit

ER1507 Catalogue No.
E2(Estradiol) ELISA Kit
Reactivity
Rat
Range
12.5-800pg/ml
Sensitivity
7.5pg/ml
Product Name
Rat E2(Estradiol) ELISA Kit
Catalogue No.
ER1507
Alias
E2(Estradiol) ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
E2 ELISA Kit allows for the in vitro quantitative determination of E2 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Rat
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-9992
EDTA plasma(n=5)86-10095
heparin plasma(n=5)88-10594
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-104%87-98%86-105%88-93%
EDTA plasma(n=5)82-95%84-98%86-101%82-100%
heparin plasma(n=5)85-98%83-100%80-90%83-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only