TUNEL Apoptosis Kits

TUNEL Apoptosis Kits

Apoptosis refers that genetic control of cells sre subject to the active and physiological process of death following their programming. It's involved in roles of gene in activation, expression, and regulation, and also has great effects on defense and immune response of organisms as well as potential therapeutic significance.

Apoptosis includes early, middle, and late phase. During early apoptosis, phosphatidylserine(PS) is exposed to the surface of cell membrane and can be detected by Annexin V apoptosis kits. During late apoptosis, DNA is broken, using TUNEL apoptosis kits.

Advantages

FITC/ FineTest®488 /555/594/647

one step, saving time and effort

detect apoptosis at single-cell level

self-developed core component, stable quality

free of potassium dimethylarsinate, safe and non-toxic to humans and the environment

cell sample(cell smears, cell slides, suspension cell),
tissue sample(paraffin and frozen section)
Experimental Principle

TUNEL refers to TdT-mediated dUTP Nick-End Labeling.

During apoptosis, chromosomal DNA is broken, producing a large number of adhesive 3'-OH terminal. These exposed 3'-OH can link with fluorescence-labeled dUTP by the catalysis of TdT. Perform the detection to show features of apoptosis via fluorescence microscope or flow cytometer .

TUNEL is suitable for various samples, including cell samples(cell smears, cell slides, suspension cell) and tissue sample(paraffin and frozen section). So TUNEL is widely applied in apoptosis research.

TUNEL-assay
Experimental Procedure

For detailed operation steps, please refer to the flow chart below:

TUNEL-flow-chart

Validation
FNCK100
Cat.No: FNCK100
FNCK101
Cat.No: FNCK101
FNCK102
Cat.No: FNCK102
FNCK103
Cat.No: FNCK103
FNCK104
Cat.No: FNCK104
Recommended TUNEL Apoptosis Kits
Cat.No Product Name Size
FNCK100 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) 20T/50T/100T
FNCK101 One-step TUNEL In Situ Apoptosis Kit (Green, FineTest®488) 20T/50T/100T
FNCK102 One-step TUNEL In Situ Apoptosis Kit (Red, FineTest®594) 20T/50T/100T
FNCK103 One-step TUNEL In Situ Apoptosis Kit (Red, FineTest®647) 20T/50T/100T
FNCK104 One-step TUNEL In Situ Apoptosis Kit (Red, FineTest®555) 20T/50T/100T
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