Annexin V Apoptosis Kits

Annexin V is the common reagent for detecting apoptosis. The probe is a calcium-dependent phospholipid binding protein with 35kDa. Specifically bind with phosphatidylserine(PS) in the cell membrane with high affinity.

The nuclear dye(PI/7-AAD) assists to conduct apoptosis detection, efficiently distinguishing early and late apoptosis.

Advantages

01High Accuracy

02High Stability

03Easy Operation

04Fast Delivery

Without cell sample fixation;

Obvious negative-positive grouping

Stored at 2-8 ℃ for 12months;

less than 2% decrease of activity

One or two step assay;

about 15min staining

Self-developed;

fast delivery, in-stock products

 

05More cost-effective

 

 

1 assay is equivalent to 2 assays;

Detection Results:

Flow cytometry analysis with Jurkat cells (1*10^6),

treated with 5 μM camptothecin for 4 hours,

stained with K019 1 assay (Left) and 0.5 assay (Right) respectively.

10^6 Cells 1-assay 0.5-assay

Annexin V is the common reagent for detecting apoptosis. The probe is a calcium-dependent phospholipid binding protein with 35kDa. Specifically bind with phosphatidylserine(PS) in the cell membrane with high affinity.

AnnexinⅤ labeled with FITC or EGFP is used as fluorescence probe. Detect apoptosis with the flow cytometer or fluorescence microscope.

apoptosis

List of Annexin V Apoptosis Kits

Cat.No Product Name Size
K018 Annexin V-EGFP/PI Apoptosis Kit 20T, 50T, 100T
K019 Annexin V-FITC/PI Apoptosis Kit 20T, 50T, 100T
K070 Annexin V-EGFP/7-AAD Apoptosis Kit 20T, 50T, 100T
K071 Annexin V-FITC/7-AAD Apoptosis Kit 20T, 50T, 100T
K072 Annexin V-APC/7-AAD Apoptosis Kit 20T, 50T, 100T
K073 Annexin V-PE/7-AAD Apoptosis Kit 20T, 50T, 100T
K074 Annexin V-FineTest®488/PI Apoptosis Kit 20T, 50T, 100T
K075 Annexin V-FineTest®488/7-AAD Apoptosis Kit 20T, 50T, 100T
K076 Annexin V-FineTest®647/PI Apoptosis Kit 20T, 50T, 100T
K077 Annexin V-FineTest®647/7-AAD Apoptosis Kit 20T, 50T, 100T
K078 Annexin V-APC/PI Apoptosis Kit 20T, 50T, 100T

Validated Images

Flow cytometry analysis with Jurkat cells (1*10^6), treated(figure 1-2) or untreated(figure 3-4) with 5 μM camptothecin for 4 hours. Stained with FineTest reagents.

FITC-H EGFP-H
Figure 1
Figure 2
FITC-H-2 EGFP-H-2
Figure 3
Figure 4
Products Comparison

Jurkat cells (5x10) treated with 5 μM camptothecin for 4 hours. Detect with FineTest and other brand's kits. The grouping of our Annexin V Apoptosis Kit is more obvious.

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