Loading control antibodies are widely used in WB to detect loading control protein expression level and ensure accuracy of experimental results by correcting the error during protein quantitation and sample loading. Besides, loading control can be used as blank control to detect protein transmembrane situation, the staining or ECL process of western blot.
FineTest provides a complete set of polyclonal and monoclonal loading control antibodies with high specificity and wide applications, which can be used in most qualitative, quantitative and localization analysis of proteins. We provide common and rare species loading control antibodies(e.g .human, mouse, rat etc) as well as custom services.
View All Loading Control Antibodies
How to Choose Loading Control Antibodies?
01 For an obvious band effect, loading control protein needs to be at least 5 KD different from target protein.
02 Loading control proteins have different expression levels according to different cell organs. It also needs to consider sample type while choosing suitable loading control antibodies.
| Sample Type | Loading Control | Rabbit pAb | Mouse mAb | MW(kDa) |
|---|---|---|---|---|
| whole-cell, cytoplasm | Beta Tubulin | FNab00869 | FNab00873 | 42 |
| alpha Tubulin | FNab00319 | |||
| GAPDH | FNab03342 | FNab03343 | 36 | |
| Beta Tubulin | FNab00880 | FNab09105 | 50-55 | |
| alpha Tubulin | FNab00333 | FNab00335 | ||
| vinculin (for high MW) | FNab09799 | FNab09800 | 117 | |
| nucleus | Lamin B1 | FNab04682 | FNab04683 | 66 |
| TBP | FNab10263 | FNab08527 | 38 | |
| YY1 | FNab10027 | 65-70 | ||
| Histone H3 | FNab03888 | 18 | ||
| PCNA | FNab06215 | FNab06217 | 38 | |
| HDAC1 | FNab03792 | FNab03793 | 55 | |
| cell membrane | ATP1A1 | FNab00691 | 100-110 | |
| mitochondrion | VDAC1 | FNab09826 | 31 | |
| COXIV | FNab01912 | FNab01914 | 17-20 | |
| endoplasmic reticulum | calreticulin | FNab01224 | 55-60 | |
| Golgi apparatus | GM130 | FNab03558 | 130 | |
| lysosomes | LAMP2 | FNab04686 | FNab09834 | 100-120 |
| blood | Transferrin | FNab08929 | FNab08930 | 77 |
03 Loading control proteins’ MW and expression level in different cell organs.
Loading control protein expression level in different mouse tissues.
| Brain | Spleen | Skeletal Muscle |
Kidney | Pancreas | Testis | Skin | Eye | Heart | Lung | |
|---|---|---|---|---|---|---|---|---|---|---|
| a-Tubulin |  |
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Low | |
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| β-Actin | |
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Low | |
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Low | |
| β-Tubulin | |
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Low | |
Low | |
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| COX IV | |
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| GAPDH | |
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| Histone H3 | |
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Low | Low | |
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| β3-Tubulin | |
Low | |
Low | Low | |
Low | |
Low | |
| 01 | Labeled loading control：add HRP-conjugated loading control antibody during incubating secondary antibodies and follow normal steps. |
| 02 | Common loading control：When molecular weight differences between target protein and selected loading control protein is low, conduct incubation, staining and detection for target protein antibody first. Then, wash antibodies on the membrane with Strip buffer. Repeat incubation, staining and detection. |
| 03 | When molecular weight differences between target protein and selected loading control protein is high, pre-stain after membrane transfer. According to the size of protein marker, cut the membrane into two parts: high and low molecular weight. Separate the loading control protein from the target protein. |
Advantages of FineTest Antibodies
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A Complete Set Of Loading Control Antibodies (MW: 15kDa - 130kDa) |
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Strict QC, Various Validations, High Performance |
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Flexible Size |
Recommended Loading Control Antibodies
|
Catalogue No. |
Product Name |
MW(kDa) |
Antibody Type |
Applications |
|
anti- beta actin antibody |
42 |
Rabbit pAb |
ELISA, IF, WB, IHC |
|
|
anti- beta actin antibody |
42 |
Mouse mAb |
ELISA, WB, IHC, IF |
|
|
anti- GAPDH antibody |
36 |
Rabbit pAb |
ELISA, IHC, WB |
|
|
anti- GAPDH antibody |
36 |
Mouse mAb |
ELISA, IHC, WB, IF, FC, IP |
|
|
anti- Beta Tubulin antibody |
50-55 |
Rabbit pAb |
ELISA, WB, IF, IP, IHC, FC |
|
|
anti- Tubulin-beta antibody |
50-55 |
Mouse mAb |
ELISA, WB, IHC, IF, IP |
Citations
- Article Title: Anti-hyperuricemic effects of three theaflavins isolated from black tea in hyperuricemic mice
IF: 3.197Journal Title: Journal of Functional FoodsTarget: GAPDH, URAT1, OAT1, GLUT9
- Article Title: lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer
IF: 3.046Journal Title: OncoTargets and TherapyTarget: GAPDH, RtcB
- Article Title: miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
IF: 2.482Journal Title: Biological ResearchTarget: beta actin
- Article Title: Inhibition of NLRP3 and Golph3 ameliorates diabetes-induced neuroinflammation in vitro and in vivo
IF: 5.955Journal Title: AgingTarget: GOLGA2/GM130, GOLPH3, Vimentin