Products

Human IL-6(Interleukin 6) ELISA Kit

EH0201 Catalogue No.
IL-6(Interleukin 6)|IL6|BSF2|HGF|HSF|IFNB2|CDF ELISA Kit
Reactivity
Human
Range
4.688-300pg/ml
Sensitivity
2.813pg/ml
Product Name
Human IL-6(Interleukin 6) ELISA Kit
Catalogue No.
EH0201
Alias
IL-6(Interleukin 6)|IL6|BSF2|HGF|HSF|IFNB2|CDF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.688-300pg/ml
Sensitivity
< 2.813pg/ml
Species
Human
UniProt ID
P05231
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)92-10399
EDTA plasma(n=5)87-10494
heparin plasma(n=5)90-10496
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-105%85-91%93-101%90-104%
EDTA plasma(n=5)82-99%82-100%86-94%84-99%
heparin plasma(n=5)85-100%80-98%80-91%81-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only