Ready-to-use enzyme-linked immunosorbent assay kits (ELISA Kits) are FineTest most trusted product line. 3,000+ hot-selling high-sensitivity ELISA kits cover about 16 species not only regular species like Human, Rat, Mouse, but also rare species like Bovine, Chicken, Canine...etc. You can choose the best appropriate ELISA type as you want, including sandwich ELISA kit, competitive ELISA kit, indirect ELISA kit. Over 3,300 scholar articles published in international well-known journals cite our ELISA products.
Search Your ELISA Kit
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R & D capability for proteins and antibodies production, self-produced raw materials, controllable quality; |
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Self-established cell bank, blood tissue sample bank, providing various samples for detection; |
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GMP-standard workshop, maintaining the clean production environment and improving product reliability; |
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Unique signal amplification technology, flexibly adjusting the kit's sensitivity and improving customers' sample detection rate; |
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High-precision production and strict QC, ensuring CV<5% with various sample detections; |
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Unique stabilized technology, maintaining the one-month activity even stored at room temperature and facilitating transportation and use. |
01 Sensitivity
For low concentration of the detection target, please select high sensitivity ELISA kit. Sensitivity corresponds to 3 times the standard deviation of the blank.
FineTest® has the unique signal amplification technology, which can flexibly adjust the kit's sensitivity. The highest sensitivity can achieve fg/ml to improving customers' sample detection rate.
02 Specificity
Judge the ability of other molecules binding with kit's antibody. Lower cross-reaction shows higher specificity. Usually, cross-reaction should not happen among the kit, the protein in the same species and family, common factors(e.g. heterophilic antibody, rheumatoid factor etc.) in the same protein serum of other species, and target protein-interacting proteins. Three strategies for improving specificity:
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Raw material: selected polyclonal and monoclonal antibody pair; |
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Reaction solution system: optimize coated buffer, sample dilution buffer and antibody dilution buffer to resist matrix effects; |
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2.3 |
Optimized detection sample: The ability of natural antigen and recombinant proteins binding with capture antibody has an |
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effect on detection value due to changes in protein conformation after binding. |
03 Precision
Also called reproducibility, the parameter for evaluating errors during repeated assay for the same sample. Intra and inter CV should be less than 10%.
FineTest® uses the machinary production, excluding artificial instability. Conduct the sample loading with electronic pipettor and automated microplate coating machine. Strictly control intra and inter CV.
04 Recovery
Usually, measure the recovery by spike-in. The recovery between 80%-120% is acceptable. Measure by adding standard into natural sample mechanism. Set the natural sample matrix without standard as blank control. Recovery is the ratio between measured target concentration and added standard concentration, calculated with percentage. Higher or lower recovery will produce false-positive or negative results and affects the detection accuracy.
| Matix | Recovery Range (%) | Average (%) |
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| Serum(n=5) | 85-98 | 93 |
| EDTA Plasma(n=5) | 88-100 | 95 |
| Heparin Plasma(n=5) | 86-98 | 93 |
05 Linearity
Add suitable concentration of standard, and dilute at 2x, 4x and 8x respectively. Measure the relevant recovery under each concentration. The recovery between 80%-120% is acceptable.
| Sample | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| Serum(n=5) | 82-98% | 84-100% | 82-100% |
| EDTA Plasma(n=5) | 80-104% | 90-102% | 86-97% |
| Heparin Plasma(n=5) | 82-95% | 83-98% | 84-100% |
06 Stability
Usually, validate by accelerated destructive experiments. Store the sealed ELISA kit at 37℃ and 2-8℃ for a long time to examine deviation of various performance indicators. Less than 10% deviation is acceptable. The better stability shows the longer storage time.
FineTest® developed a stabilized system, which can store the ELISA kit at 40℃ for one week. The activity is still higher than 95%. Most ELISA kits can be stored at 4℃ for 2 years. The activity can still have 85-95%, basically not affecting the kit's working and accurate results.
07 Natural sample testing
Finally, ELISA kits can detect natural sample and suitable sample type. Judge sample type according to the property of target in advance, and detect a mimimum of 5 samples. Provide sample data to our customers when necessary.
FineTest ELISA Kits Cited in Publications
FineTest ELISA products have helped global researchers conduct their bioassays. Over 3,400 citations show the high sensitivity and reproducibility of our kits, including leading journals like Cell, Cell Research, Immunity, Journal of Medical Virology, Nature Communications etc.
Cell IF: 31.398
Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity
- Cat.No
- Product Name
- EH4233
- Human PGE2(Prostaglandin E2) ELISA Kit
Cell Research IF: 46.351
- Cat.No
- Product Name
- EH4343
- Human SCUBE2(Signal peptide, CUB and EGF-like domain-containing protein 2) ELISA Kit
- EM1360
- Mouse SHH(Hedgehog Homolog, Sonic) ELISA Kit
Annals of the Rheumatic Diseases IF: 28.003
- Cat.No
- Product Name
- EH3723
- Human RNASET2(Ribonuclease T2) ELISA Kit
Advanced Materials IF: 29.4
- Cat.No
- Product Name
- EH4514
- Human PD1/PDL1 Inhibitor Screening Assay Kit
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