Products

Rat IL-10(Interleukin-10) ELISA Kit

ER0033 Catalogue No
Reactivity
Rat
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Rat IL-10(Interleukin-10) ELISA Kit
Catalogue No.
ER0033
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
P29456
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-9994
EDTA plasma(n=5)85-10497
heparin plasma(n=5)89-10496
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-102%96-105%92-100%85-104%
EDTA plasma(n=5)83-98%90-101%82-101%86-99%
heparin plasma(n=5)82-91%89-100%85-98%81-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only