Products

Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

EH0302 Catalogue No
TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Reactivity
Human
Range
15.6-1000pg/ml
Sensitivity
1pg/ml
Product Name
Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No.
EH0302
Alias
TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TNFα ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.6-1000pg/ml
Sensitivity
< 1pg/ml
Species
Human
UniProt ID
P01375
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)95-105100
EDTA plasma(n=5)91-9894
heparin plasma(n=5)85-10493
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)93-103%88-101%88-96%93-104%
EDTA plasma(n=5)91-101%82-94%82-100%82-99%
heparin plasma(n=5)81-98%85-97%83-99%80-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only