- Product Name
- Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit
- Catalogue No.
- TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- TNFα ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in plasma, tissue homogenates and other biological fluids.
- < 1pg/ml
- UniProt ID
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 95-105 100 EDTA plasma(n=5) 91-98 94 heparin plasma(n=5) 85-104 93
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 93-103% 88-101% 88-96% 93-104% EDTA plasma(n=5) 91-101% 82-94% 82-100% 82-99% heparin plasma(n=5) 81-98% 85-97% 83-99% 80-100%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area