The coronavirus, named for its coronal form, was first identified in the 1960s and can cause a range of respiratory symptoms. Coronavirus such as Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV) can aggravate human symptoms and cause pneumonia, severe acute respiratory syndrome and death. SARS-CoV-2 (2019-nCoV) is the newly discovered coronavirus strain.
Chinese scientists have determined that novel coronavirus belongs to 2B CoV subgroup and is different from sars-cov. Genome sequencing showed that the sequence homology of SARS-CoV-2 and SARS-CoV was 79.5%, and that of SARS-CoV-2 and bat coronavirus was 96% [1].
Coronaviruses invade host cells by attaching spike proteins to specific host cell receptors. SARS-CoV uses the angiotensin-converting enzyme II(ACE2) host cell receptor, while MERS-CoV uses CD26(DPP4) [2]. Recent results indicate that SARS-CoV-2 can enter cells using the same receptor ACE2 as SARS-CoV [1.3.4]. More studies are needed to confirm ACE2 is the only way to infect the host for SARS-CoV-2. After SARS-CoV-2 enters the cell through ACE2, Cathepsin degrades Spike protein to release nucleic acid. Some studies have shown that using Cathepsin B/L inhibitors can block the process. The study also showed that SARS-CoV-2 could also be directly hydrolyzed by the transmembrane protein TMPRSS2, enter and release nucleic acids [5]。AI was used to screen the inhibitors (Baricitinib, Sunitinib, Erlotinib, etc.) of host endocytic regulatory factor AAK1, the inhibitors can block the delivery of virus to cells and the intracellular assembly of virus particles by inhibiting AAK1[6]。
FineTest® can provide a variety of tools for coronavirus (SARS-CoV-2) research, including human anti- SARS-CoV-2 antibody (IgG, IgM), inflammatory cytokines, to help the scientific community understand the mechanisms of infection and develop effective treatments.
SARS-CoV-2 Antibody Qualitative ELISA Kits
| Product Name | Cat.No. | Application |
| Human Anti-2019 nCoV(N) IgG ELISA Kit(Qualitative) | EH4395 | ELISA |
| Human Anti-2019 nCoV(N) IgM ELISA Kit(Qualitative) | EH4396 | ELISA |
| Human Anti-2019 nCoV(S) IgG ELISA Kit(Qualitative) | EH4941 | ELISA |
| Human Anti-2019 nCoV(S) IgM ELISA Kit(Qualitative) | EH4942 | ELISA |
| Human Anti-2019 nCoV(N+S) IgM ELISA Kit(Qualitative) | EH4948 | ELISA |
| Human Anti-2019 nCoV(N+S) IgG ELISA Kit(Qualitative) | EH4946 | ELISA |
| Anti-2019 nCoV(N) Ig ELISA Kit(Qualitative) | EU3125 | ELISA |
SARS-CoV-2 Antibody Quantitative ELISA Kits
| Product Name | Cat.No. | Application |
| Human Anti-SARS-CoV2(N) IgG ELISA Kit(Quantitative) | EH4397 | ELISA |
| Human Anti-SARS-CoV2(N) IgM ELISA Kit(Quantitative) | EH4940 | ELISA |
| Human Anti-2019 nCoV (S) IgM ELISA Kit(Quantitative) | EH4951 | ELISA |
| Mouse Anti-2019-nCoV(S) IgA ELISA Kit(Quantitative) | EM1906 | ELISA |
| Mouse Anti-2019-nCoV(S) IgG ELISA Kit(Quantitative) | EM1907 | ELISA |
| Human Anti-SARS-CoV2(S) IgG ELISA Kit(Quantitative) | EH4981 | ELISA |
SARS-CoV-2 Antigen ELISA Kits
| Product Name | Cat.No. | Application |
| COVID-19 nucleoprotein ELISA Kit(Quantitative) | EU3124 | ELISA |
| 2019 nCoV(S) ELISA Kit(Quantitative) | EU3128 | ELISA |
SARS-CoV-2 Specific Antibodies
| Product Name | Cat.No. | Application |
| Anti- 2019 nCov N protein antibody(Clone#1G5) | FNab10308 | ELISA, Colloidal Gold-Based, WB |
| Anti- 2019 nCov N protein antibody(Clone#3G6) | FNab10309 | ELISA, Colloidal Gold-Based, WB |
| Anti- 2019 nCOV S1 protein antibody | FNab10400 | ELISA, WB |
SARS-CoV-2 Proteins
| Product Name | Cat.No. | Application |
| Recombinant Nucleoprotein [2019-Novel Coronavirus] | P3003 | ELISA, WB |
| Recombinant Spike Glycoprotein RBD [2019-Novel Coronavirus] | P3006 | ELISA, WB |
Sample test results（EH4397）
Samples came from rehabilitation clients (1-2 months after recovery) of mobile cabin hospital. The plasma samples were diluted 1:100. TMB Color development time was 10 minutes at 37°C.Due to individual differences, it is recommended to dilute the sample 1:50-1:2000.
| Rehabilitation clients (ug/ml) | Healthy volunteers(ug/ml) | ||||||
| 1# | 31 | 5# | 35 | 1# | ＜0.1 | 5# | not detect |
| 2# | 7 | 6# | 17 | 2# | not detect | 6# | not detect |
| 3# | 24 | 7# | 38 | 3# | not detect | 7# | not detect |
| 4# | 47 | 8# | 0.6 | 4# | not detect | 8# | not detect |
Sample test results（EH4940）
Patients (1 month after recovery) came from mobile cabin hospital. Plasma sample dilution: 1:100-1:500.
| Patients (ug/ml) | Healthy volunteers(ng/ml) | ||||||
| 1# | 7 | 5# | 5.7 | 1# | not detect | 5# | not detect |
| 2# | 2.4 | 6# | 2.3 | 2# | not detect | 6# | not detect |
| 3# | 12 | 7# | 1.6 | 3# | not detect | 7# | ＜0.1 |
| 4# | 6 | 8# | 2.1 | 4# | ＜0.2 | 8# | not detect |
Sample test data（EH4395）
Samples came from rehabilitation clients (1-2 months after recovery) of mobile cabin hospital. The plasma samples were diluted 1:50. TMB Color development time was 10 minutes at 37°C. NCx=0.197
| Rehabilitation clients(OD450) | Healthy volunteers(OD450) | ||||||
| 1# | 3.422 | 9# | 2.458 | 1# | 0.245 | 9# | 0.217 |
| 2# | 1.504 | 10# | 2.164 | 2# | 0.203 | 10# | 0.199 |
| 3# | 2.334 | 11# | 1.832 | 3# | 0.188 | 11# | 0.206 |
| 4# | 3.188 | 12# | 1.945 | 4# | 0.232 | 12# | 0.237 |
| 5# | 1.926 | 13# | 3.023 | 5# | 0.174 | 13# | 0.164 |
| 6# | 2.689 | 14# | 2.861 | 6# | 0.182 | 14# | 0.222 |
| 7# | 3.021 | 15# | 1.639 | 7# | 0.168 | 15# | 0.163 |
| 8# | 2.578 | 16# | 2.369 | 8# | 0.159 | blank | 0.058 |
Sample test data（EH4396）
Samples came from patients (1 month after recovery) of mobile cabin hospital. The plasma samples were diluted 1:50. TMB Color development time was 10 minutes at 37°C.NCx=0.194
| Patients (OD450) | Healthy volunteers(OD450) | ||||||
| 1# | 1.735 | 5# | 2.108 | 1# | 0.224 | 5# | 0.230 |
| 2# | 0.881 | 6# | 1.544 | 2# | 0.189 | 6# | 0.157 |
| 3# | 1.852 | 7# | 1.247 | 3# | 0.207 | 7# | 0.164 |
| 4# | 1.353 | 8# | 1.115 | 4# | 0.185 | blank | 0.057 |
ACE2：host receptor of SARS-CoV-2, SARS-CoV
| Product Name | Cat.No. | Application | Host |
| Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit | EH0027 | ELISA | Human |
| Human CD147(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit | EH0073 | ELISA | Human |
| Rat Ace2(Angiotensin-converting enzyme 2) ELISA Kit | ER0609 | ELISA | Rat |
| anti- ACE2 antibody | FNab00081 | ELISA, WB, IP | Human, Mouse, Rat |
DPP4：host receptor of SARS-CoV
| Product Name | Cat.No. | Application | Host |
| anti- DPP4 antibody | FNab02520 | ELISA, WB, IHC | Human, Hamster |
| Human CD26(Dipeptidyl peptidase 4) ELISA Kit | EH0003 | ELISA | Human |
| Mouse CD26(Dipeptidyl peptidase 4) ELISA Kit | EM1632 | ELISA | Mouse |
| Rat DPPⅣ(Dipeptidyl Peptldase Ⅳ) ELISA Kit | ER0910 | ELISA | Rat |
Cathepsin B：May participate in the hydrolysis of coronavirus S protein, thereby releasing nucleic acids
| Product Name | Cat.No. | Application | Host |
| anti- Cathepsin B antibody | FNab01304 | ELISA, WB, IHC, IF, FC | Human, Mouse, Rat |
| Human CTSB(Cathepsin B) ELISA Kit | EH0051 | ELISA | Human |
| Rat Ctsb(Cathepsin B) ELISA Kit | ER0294 | ELISA | Rat |
Cytokine products for human coronavirus research
| Product Name | Cat.No. | Application | Host |
| anti- IL1 beta antibody | FNab04209 | ELISA, IHC, WB | Human, Mouse |
| Human IL-2(Interleukin 2) ELISA Kit | EH0189 | ELISA | Human |
| Human IL-6(Interleukin 6) ELISA Kit | EH0201 | ELISA | Human |
| anti- IL-6 antibody | FNab04283 | ELISA, IHC, WB | Human |
| anti- IL-6 antibody | FNab04282 | ELISA, WB, IHC | Human, Mouse, Rat |
| anti- IL-17 antibody | FNab04225 | ELISA, WB, FC | Human |
| Human IFNγ(Interferon gamma) ELISA Kit | EH0164 | ELISA | Human |
| anti- Interferon gamma antibody | FNab04358 | ELISA, IHC, WB | Human, Mouse |
| Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit | EH0302 | ELISA | Human |
| anti- TNF alpha antibody | FNab09822 | ELISA, WB, IHC | Human, Mouse, Rat |
| Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit | EH0327 | ELISA | Human |
| anti- VEGF antibody | FNab09933 | ELISA, WB, IHC | Human |
| Human TGFβ1(Transforming Growth Factor Beta 1) ELISA Kit | EH0287 | ELISA | Human |
| Human CRP(C-Reactive Protein) ELISA Kit | EH0099 | ELISA | Human |
| Human PCT(Procalcitonin) ELISA Kit | EH0341 | ELISA | Human |
| Human SAA(Serum Amyloid A) ELISA Kit | EH0336 | ELISA | Human |
| Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit | EH0222 | ELISA | Human |
If you are interested in the commercial use of our products, please contact us at fine@fn-test.com.
References
[1]Shi, Z.L., et al. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. bioRxiv 2020.
[2]Xintian Xu et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. SCIENCE CHINA Life Sciences.
[3]Xu, X., et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. Sci. China Life Sci. 2020.
[4]Letko, M.C., Munster, V. Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV. bioRxiv 2020.