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FineTest®—Tools for SARS-CoV-2 Research

The coronavirus, named for its coronal form, was first identified in the 1960s and can cause a range of respiratory symptoms. Coronavirus such as Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV) can aggravate human symptoms and cause pneumonia, severe acute respiratory syndrome and death. SARS-CoV-2 (2019-nCoV) is the newly discovered coronavirus strain.

Chinese scientists have determined that novel coronavirus belongs to 2B CoV subgroup and is different from sars-cov. Genome sequencing showed that the sequence homology of SARS-CoV-2 and SARS-CoV was 79.5%, and that of SARS-CoV-2 and bat coronavirus was 96% [1].

Coronaviruses invade host cells by attaching spike proteins to specific host cell receptors. SARS-CoV uses the angiotensin-converting enzyme II(ACE2) host cell receptor, while MERS-CoV uses CD26(DPP4) [2]. Recent results indicate that SARS-CoV-2 can enter cells using the same receptor ACE2 as SARS-CoV [1.3.4]. More studies are needed to confirm ACE2 is the only way to infect the host for SARS-CoV-2. After SARS-CoV-2 enters the cell through ACE2, Cathepsin degrades Spike protein to release nucleic acid. Some studies have shown that using Cathepsin B/L inhibitors can block the process. The study also showed that SARS-CoV-2 could also be directly hydrolyzed by the transmembrane protein TMPRSS2, enter and release nucleic acids [5]。AI was used to screen the inhibitors (Baricitinib, Sunitinib, Erlotinib, etc.) of host endocytic regulatory factor AAK1, the inhibitors can block the delivery of virus to cells and the intracellular assembly of virus particles by inhibiting AAK1[6]。

FineTest® can provide a variety of tools for coronavirus (SARS-CoV-2) research, including human anti- SARS-CoV-2 antibody (IgG, IgM), inflammatory cytokines, to help the scientific community understand the mechanisms of infection and develop effective treatments.

SARS-CoV-2 specific antibodies ,proteins and ELISA KITS

Product Name Cat.No. Application
Recombinant Nucleoprotein [2019-Novel Coronavirus] P3003 ELISA, WB
anti- 2019 nCOV N protein antibody(clone 1G5) FNab10308 ELISA, ColloidalGold-Based, WB
anti- 2019 nCOV N protein antibody(clone 3C6) FNab10309 ELISA, ColloidalGold-Based, WB
Mouse anti- human IgG FNab10307 ELISA, IHC, WB, ColloidalGold-Based
Mouse anti- human IgM FNab10306 ELISA, IHC, WB, ColloidalGold-Based
poly-HRP conjugated Mouse anti- human IgG FNSA-0114 ELISA
poly-HRP conjugated Mouse anti- human IgM FNSA-0113 ELISA
Human anti-2019 nCoV(N) IgG ELISA Kit(qualitative) EH4395 ELISA
Human anti-2019 nCoV(N) IgM ELISA Kit(qualitative) EH4396 ELISA
Human anti-SARS-CoV2 IgG ELISA Kit(quantitative) EH4397 ELISA
Human anti-SARS-CoV2 IgM ELISA Kit(quantitative) EH4940 ELISA
Human anti-2019 nCoV(S) IgG ELISA Kit(qualitative) EH4941 ELISA
Human anti-2019 nCoV(S) IgM ELISA Kit(qualitative) EH4942 ELISA
COVID-19 nucleoprotein ELISA Kit(quantitative) EU3124 ELISA
Anti-2019 nCoV(N) Ig ELISA Kit(qualitative) EU3125 ELISA
2019-nCoV IgMIgG Antibody Colloidal Gold Assay Kit EH4933 Colloidal Gold Assay Kit
2019-Novel Coronavirus(2019-nCoV) RT-qPCR Detection Kit EH4934 RT-qPCR Detection Kit

 

Sample test results(EH4397)

Samples came from rehabilitation clients of mobile cabin hospital. The plasma samples were diluted 1:100. TMB Color development time was 10 minutes at 37℃.Due to individual differences, it is recommended to dilute the sample 1:50-1:2000.

Rehabilitation clients (ug/ml) Healthy volunteers(ug/ml)
1# 31 5# 35 1# <0.1 5# not detect
2# 7 6# 17 2# not detect 6# not detect
3# 24 7# 38 3# not detect 7# not detect
4# 47 8# 0.6 4# not detect 8# not detect

 

Sample test results(EH4940)

Patients from mobile cabin hospital. Plasma sample dilution 1:100-1:500.

Patients (ug/ml) Healthy volunteers(ng/ml)
1# 7 5# 5.7 1# not detect 5# not detect
2# 2.4 6# 2.3 2# not detect 6# not detect
3# 12 7# 1.6 3# not detect 7# <0.1
4# 6 8# 2.1 4# <0.2 8# not detect

 

Sample test data(EH4395)

Samples came from rehabilitation clients of mobile cabin hospital. The plasma samples were diluted 1:50. TMB Color development time was 10 minutes at 37℃. NCx= 0.197.

Rehabilitation clients(OD450) Healthy volunteers(OD450)
1# 3.422 9# 2.458 1# 0.245 9# 0.217
2# 1.504 10# 2.164 2# 0.203 10# 0.199
3# 2.334 11# 1.832 3# 0.188 11# 0.206
4# 3.188 12# 1.945 4# 0.232 12# 0.237
5# 1.926 13# 3.023 5# 0.174 13# 0.164
6# 2.689 14# 2.861 6# 0.182 14# 0.222
7# 3.021 15# 1.639 7# 0.168 15# 0.163
8# 2.578 16# 2.369 8# 0.159 blank 0.058

Sample test data(EH4396)

Samples came from patients of mobile cabin hospital. The plasma samples were diluted 1:50. TMB Color development time was 10 minutes at 37°C. NCx=0.194.

Patients (OD450) Healthy volunteers(OD450)
1# 1.735 5# 2.108 1# 0.224 5# 0.230
2# 0.881 6# 1.544 2# 0.189 6# 0.157
3# 1.852 7# 1.247 3# 0.207 7# 0.164
4# 1.353 8# 1.115 4# 0.185 blank 0.057

 

ACE2host receptor of SARS-CoV-2SARS-CoV

Product Name Cat.No. Application Host
Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit EH0027 ELISA Human
Human CD147(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit EH0073 ELISA Human
Rat Ace2(Angiotensin-converting enzyme 2) ELISA Kit ER0609 ELISA Rat
anti- ACE2 antibody FNab00081 ELISA, WB, IP Human, Mouse, Rat

 

DPP4host receptor of SARS-CoV

Product Name Cat.No. Application Host
anti- DPP4 antibody FNab02520 ELISA, WB, IHC Human, Hamster
Human CD26(Dipeptidyl peptidase 4) ELISA Kit EH0003 ELISA Human
Mouse CD26(Dipeptidyl peptidase 4) ELISA Kit EM1632 ELISA Mouse
Rat DPPⅣ(Dipeptidyl Peptldase Ⅳ) ELISA Kit ER0910 ELISA Rat

 

Cathepsin BMay participate in the hydrolysis of coronavirus S protein, thereby releasing nucleic acids

Product Name Cat.No. Application Host
anti- Cathepsin B antibody FNab01304 ELISA, WB, IHC, IF, FC Human, Mouse, Rat
Human CTSB(Cathepsin B) ELISA Kit EH0051 ELISA Human
Rat Ctsb(Cathepsin B) ELISA Kit ER0294 ELISA Rat

 

Cytokine products for human coronavirus research

Product Name Cat.No. Application Host
anti- IL1 beta antibody FNab04209 ELISA, IHC, WB Human, Mouse
Human IL-2(Interleukin 2) ELISA Kit EH0189 ELISA Human
Human IL-6(Interleukin 6) ELISA Kit EH0201 ELISA Human
anti- IL-6 antibody FNab04283 ELISA, IHC, WB Human
anti- IL-6 antibody FNab04282 ELISA, WB, IHC Human, Mouse, Rat
anti- IL-17 antibody FNab04225 ELISA, WB, FC Human
Human IFNγ(Interferon gamma) ELISA Kit EH0164 ELISA Human
anti- Interferon gamma antibody FNab04358 ELISA, IHC, WB Human, Mouse
Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit EH0302 ELISA Human
anti- TNF alpha antibody FNab09822 ELISA, WB, IHC Human, Mouse, Rat
Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327 ELISA Human
anti- VEGF antibody FNab09933 ELISA, WB, IHC Human
Human TGFβ1(Transforming Growth Factor Beta 1) ELISA Kit EH0287 ELISA Human
Human CRP(C-Reactive Protein) ELISA Kit EH0099 ELISA Human
Human PCT(Procalcitonin) ELISA Kit EH0341 ELISA Human
Human SAA(Serum Amyloid A) ELISA Kit EH0336 ELISA Human
Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit EH0222 ELISA Human

 

If you are interested in the commercial use of our products, please contact us at fine@fn-test.com.

References

[1]Shi, Z.L., et al. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. bioRxiv 2020.

[2]Xintian Xu et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. SCIENCE CHINA Life Sciences.

[3]Xu, X., et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. Sci. China Life Sci. 2020.

[4]Letko, M.C., Munster, V. Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV. bioRxiv 2020.