Products

Human IFNγ(Interferon gamma) ELISA Kit

EH0164 Catalogue No
IFN-γ ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit
Reactivity
Human
Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Product Name
Human IFNγ(Interferon gamma) ELISA Kit
Catalogue No.
EH0164
Alias
IFN-γ ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IFNγ ELISA Kit allows for the in vitro quantitative determination of IFNγ concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
P01579
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IFNγ and the recovery rates were calculated by comparing the measured value to the expected amount of IFNγ in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)90-10193
EDTA plasma(n=5)87-10196
heparin plasma(n=5)87-10297
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNγ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-101%86-101%87-102%85-100%
EDTA plasma(n=5)89-101%83-94%86-92%88-101%
heparin plasma(n=5)95-99%81-86%80-97%80-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only