Products
Human anti-2019-nCoV(N) IgM ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-2019 nCoV (N) IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-2019 nCoV (N) IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EH4396
- Alias:
- SARS-CoV-2 Nucleoprotein IgM ELISA Kit
- Species:
- Human
- Range:
- Qualitative
- Sensitivity:
- Qualitative
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- Product Name
- Human anti-2019-nCoV(N) IgM ELISA Kit
- Alias
- SARS-CoV-2 Nucleoprotein IgM ELISA Kit
- Catalogue No.
- EH4396
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Qualitative
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- Qualitative
- Sensitivity
- Qualitative
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with anti-2019 nCoV (N) IgM , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Wash the plate twice before adding the standard and sample.
- Step 2: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
- Washing: Wash the plate three times and immerse for 1min each time.
- Step 3: Add 100ul HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37°C.
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
- Journal:
- Journal of Genetic Engineering and Biotechnology
- Author:
- Research Group Immune- and Bio-markers for Infection, the Center of Excellent for Advanced Science, The National Research Centre, 12622 Cairo, Egypt
- Sample:
- serum
- Cited Date:
- 2024-08-09
- Product:
- Journal:
- Current Microbiology
- Author:
- Research Group Immune-and Bio-Markers for Infection, The Centre of Excellence for Advanced Science, The National Research Centre, Giza, 12622, Egypt
- Cited Date:
- 2023-12-08
- Product:
- Journal:
- Viral Immunology
- Author:
- Department of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute, The National Research Centre, Cairo, Egypt
- Cited Date:
- 2023-11-17
- Product: