Human anti-2019-nCoV(N) IgM ELISA Kit

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-2019 nCoV(N) IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-2019 nCoV(N) IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
EH4396
Alias: 
anti-2019 nCoV ELISA Kit
Species: 
Human
Range: 
Qualitative
Sensitivity: 
Qualitative
  • SPECIFICATIONS
  • CITATIONS
Product Name
Human anti-2019-nCoV(N) IgM ELISA Kit
Alias
anti-2019 nCoV ELISA Kit
Catalogue No.
EH4396
Size
48T/96T
Species
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Qualitative
Detection Wavelength
OD450
Reaction Duration
3 hours
Range
Qualitative
Sensitivity
Qualitative
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with anti-2019 nCoV(N) IgM , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Assay Procedure Summary
Elisa实验原理图
  • Step 1: Wash the plate twice before adding the standard and sample.
  • Step 2: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
  • Washing: Wash the plate three times and immerse for 1min each time.
  • Step 3: Add 100ul HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37°C.
  • Washing: Wash the plate five times and immerse for 1min each time.
  • Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
  • Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
IF: 2.6
Journal:
Current Microbiology
Author:
Research Group Immune-and Bio-Markers for Infection, The Centre of Excellence for Advanced Science, The National Research Centre, Giza, 12622, Egypt
Cited Date:
2023-12-08
Product:
IF: 2.2
Journal:
Viral Immunology
Author:
Department of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute, The National Research Centre, Cairo, Egypt
Cited Date:
2023-11-17
Product: