Products
COVID-19 Spike Protein(RBD) ELISA Kit
- Product Name
- COVID-19 Spike Protein(RBD) ELISA Kit
- Alias
- COVID-19-S（RBD） ELISA Kit
- Catalogue No.
- EU3128
- Size
- 48T/96T
- Species
- Coronavirus(COVID-19, SARS-CoV-2, 2019-nCoV, Novel Coronavirus Spike Glycoprotein,RBD, SARS-CoV-2 S1)
- UniProt No.
- P0DTC2
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 23.438-1500pg/ml
- Sensitivity
- 14.063pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with COVID-19-S(RBD) , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.06 0.063 0.061 23.438 0.16 0.168 0.163 46.875 0.272 0.286 0.278 93.75 0.47 0.494 0.48 187.5 0.725 0.762 0.74 375 1.235 1.298 1.26 750 1.787 1.878 1.823 1500 2.061 2.166 2.103 
- Recovery
-
Add a certain amount of COVID-19-S(RBD) into the sample. Calculate the recovery by comparing the measured value with the expected amount of COVID-19-S(RBD) in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 85-103 92 EDTA plasma(n=10) 89-101 97 Heparin plasma(n=10) 86-104 99 - Linearity
-
Dilute the sample with a certain amount of COVID-19-S(RBD) at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 90-101% 89-102% 85-97% EDTA plasma(n=10) 83-91% 82-97% 82-96% Heparin plasma(n=10) 84-99% 88-99% 85-99% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 46.42 194 756 45.91 187 748 Standard deviation 2.3 11.52 39.54 2.85 8.75 38 CV(%) 4.95 5.94 5.23 6.2 4.68 5.08 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
