Products

Anti- VEGF antibody

FNab09933 Catalogue No.
Synonyms
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Host
Mouse
Reactivity
Human
Isotype
IgG2b
Tested Application
ELISA, WB, IHC
Product Information
Product Name
Anti- VEGF antibody
Catalogue No.
FNab09933
Size
100µg
Form
liquid
Purification
protein A+G purified
Purity
≥95% as determined by SDS-PAGE
Host
Mouse
Clonality
monoclonal
Isotype
IgG2b
Storage
0.01MPBS pH 7.4,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010). This antibody can recognize all the isoforms of VEFGA.
Immunogen Information
Immunogen
vascular endothelial growth factor A
Synonyms
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Observed MW
45 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:1000-1:4000; IHC: 1:50-1:500
Uniprot information
UniProt ID
P15692
Images
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using FNab09933 (VEGF antibody) at dilution of 1:200
MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab09933 (VEGF antibody) at dilution of 1:2000