Products
Human anti-SARS-CoV2(N) IgM ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-SARS-CoV2 (N) IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-SARS-CoV2 (N) IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Product Name
- Human anti-SARS-CoV2(N) IgM ELISA Kit
- Alias
- Nucleoprotein IgM ELISA Kit
- Catalogue No.
- EH4940
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 1.5 hours
- Range
- 0.781-50ng/ml
- Sensitivity
- 0.469ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with anti-SARS-CoV2 (N) IgM , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.05 0.053 0.051 0.781 0.217 0.228 0.221 1.562 0.315 0.331 0.321 3.125 0.514 0.54 0.524 6.25 0.726 0.763 0.741 12.5 1.08 1.135 1.102 25 1.491 1.567 1.521 50 2.05 2.155 2.092 
- Recovery
-
Add a certain amount of anti-SARS-CoV2 (N) IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of anti-SARS-CoV2 (N) IgM in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 88-100 92 EDTA plasma(n=10) 88-92 90 Heparin plasma(n=10) 88-104 97 - Linearity
-
Dilute the sample with a certain amount of anti-SARS-CoV2 (N) IgM at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 87-103% 86-102% 90-103% EDTA plasma(n=10) 85-98% 84-101% 83-99% Heparin plasma(n=10) 80-100% 82-100% 80-96% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 1.48 6.24 24.06 1.54 5.85 23.71 Standard deviation 0.08 0.34 1.26 0.08 0.32 1.24 CV(%) 5.43 5.48 5.23 5.43 5.43 5.21 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
