Products
Mouse OVA sIgG2a(Ovalbumin Specific IgG2a) ELISA Kit
- SPECIFICATIONS
- CITATIONS
- Product Name
- Mouse OVA sIgG2a(Ovalbumin Specific IgG2a) ELISA Kit
- Alias
- ova sigg2a ELISA Kit, Ovalbumin Specific IgG2a ELISA Kit
- Catalogue No.
- EM1997
- Size
- 48T/96T
- Species
- Mouse
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Capture ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 3 hours
- Range
- 0.313-20ng/ml
- Sensitivity
- 0.188ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with OVA sIgG2a , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 HRP-Antigen(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antigen Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) OD-1 OD-2 Average 0 0.077 0.084 0.077 0.312 0.155 0.152 0.155 0.625 0.234 0.245 0.234 1.25 0.319 0.32 0.319 2.5 0.577 0.596 0.577 5 1.199 1.18 1.199 10 1.719 1.772 1.719 20 2.446 2.434 2.446 - Recovery
-
Add a certain amount of OVA sIgG2a into the sample. Calculate the recovery by comparing the measured value with the expected amount of OVA sIgG2a in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 87-102 98 EDTA plasma(n=10) 95-101 99 Heparin plasma(n=10) 93-101 96 - Linearity
-
Dilute the sample with a certain amount of OVA sIgG2a at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 83-100% 80-95% 87-95% EDTA plasma(n=10) 89-94% 81-100% 85-102% Heparin plasma(n=10) 86-101% 86-101% 83-95% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 0.65 2.53 10.21 0.59 2.37 9.82 Standard deviation 0.03 0.15 0.66 0.03 0.11 0.39 CV(%) 4.98 5.82 6.42 4.88 4.68 4.02 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
- Journal:
- Autoimmunity
- Cited Date:
- 2023-03-24
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