Loading Control Antibodies and Tag Antibodies

1. Loading Control Antibodies

Loading control antibodies are widely used in WB to detect loading control protein expression level and ensure accuracy of experimental results by correcting the error during protein quantitation and sample loading. Besides, loading control can be used as blank control to detect protein transmembrane situation, the staining or ECL process of western blot.

FineTest provides a complete set of polyclonal and monoclonal loading control antibodies with high specificity and wide applications, which can be used in most qualitative, quantitative and localization analysis of proteins. We provide common and rare species loading control antibodies(e.g .human, mouse, rat etc) as well as custom services.

2. How to Choose Loading Control Antibodies?

2.1. Molecular Weight

For an obvious band effect, loading control protein needs to be at least 5 KD different from target protein.

2.2. Expression Position

Loading control proteins have different expression levels according to different cell organs. It also needs to consider sample type while choosing suitable loading control antibodies.

 

Brain

Spleen

Skeletal Muscle

Kidney

Pancreas

Testis

Skin

Eye

Heart

Lung

α-Tubulin

Low

β-Actin

Low

Low

β-Tubulin

Low

Low

COX IV

GAPDH

Histone H3

Low

Low

β3-Tubulin

Low

Low

Low

Low

**Loading control protein expression level in different mouse tissues.

Sample Type

Loading Control

MW(kDa)

cell membrane

ATP1A1

100-110

mitochondrion

VDAC1

31

COXIV

17-20

endoplasmic reticulum

calreticulin

55-60

Golgi apparatus

GM130

130

**Loading control proteins’ MW and their expression level in different cell organs.

Sample Type

Loading Control

MW(kDa)

lysosomes

LAMP2

100-120

blood

Transferrin

77

whole-cell, cytoplasm

β-actin, α-actin

42

GAPDH

36

β-tubulin,

α-tubulin

50-55

Vinculin

(for high MW)

117

nucleus

Lamin B1

66

TBP

38

YY1

65-70

Histone H3

18

PCNA

38

HDAC1

55

3. Advantages of FineTest Antibodies

  • A complete set of loading control antibodies with wide molecular weight range from 15kDa to 130kDa;
  • Strict QC and various validations, high performance accepted by wide customers;
  • Flexible size for your choice.

4. Recommended Loading Control Antibodies

Sample Type

Product Name

MW(kDa)

Antibody Type

Catalog No.

Applications

whole-cell,

cytoplasm

anti- beta actin antibody

42

Rabbit pAb

FNab00869

ELISA, IF, WB, IHC

anti- beta actin antibody

42

Mouse mAb

FNab00873

ELISA, WB, IHC, IF

anti- GAPDH antibody

36

Rabbit pAb

FNab03342

ELISA, IHC, WB

anti- GAPDH antibody

36

Mouse mAb

FNab03343

ELISA, IHC, WB, IF, FC, IP

anti- Beta Tubulin antibody

50-55

Rabbit pAb

FNab00880

ELISA, WB, IF, IP, IHC, FC

anti- Tubulin-beta antibody

50-55

Mouse mAb

FNab09105

ELISA, WB, IHC, IF, IP

**For more loading control antibodies, please contact us for a full list.

5. Tag Antibodies

Recombinant proteins usually own a type of tag after purification. Tag antibodies which can specifically recognize tagged recombinant protein are efficient research tools for recombinant protein expression. FineTest offers various tag antibodies which have been strictly validated in applications like WB, IP, IF, FC etc.

5.1. How to Choose Tag Antibodies

Tag antibody needs to be chosen based on the target recombinant proteins’ tag. And we recommend flag tagged protein for WB and Flag, HA or cMyc tag for IP.

  • Choose tag antibodies according to proteins' tag;
  • Recommend Protein with flag tag for WB;
  • Recommend Protein with flag, HA or cMyc tag for IP

5.2. Recommended Tag Antibodies

Tag Type

Catalog No.

Applications

Product Name

Flag tag

FNab03154

ELISA, WB, IF

anti- Flag tag antibody

FNab03155

ELISA, WB, IP

anti- Flag tag antibody

His tag

FNab00008

ELISA, WB, IP, IF

anti- 6*His, His-Tag antibody

FNab00010

ELISA, WB

anti- 6*his,His tag antibody

HA tag

FNab03742

ELISA, WB, IP, IF

anti- HA tag antibody

FNab03762

ELISA, IP, WB, IF

anti- HA-Tag antibody

Myc tag

FNab05462

ELISA, WB, IP, IF

anti- MYC-tag antibody

FNab05456

ELISA, WB, IP, IF

anti- MYC tag antibody

**A List of Tag Antibodies.

Tag Type

Catalog No.

Applications

Product Name

GST tag

FNab03683

ELISA, WB

anti- GST Tag antibody

FNab03684

ELISA, WB, IP

anti- GST tag antibody

GFP tag

FNab03433

ELISA, WB, IP

anti- GFP tag antibody

FNab03434

ELISA, WB, IP, IF

anti- GFP tag antibody

MBP tag

FNab05042

ELISA, WB, IP

anti- MBP tag antibody

FNab05043

ELISA, WB

anti- MBP-Tag antibody

S1 tag

FNab07549

ELISA, WB

anti-S1 tag antibody

V5 tag

FNab09354

ELISA, WB, IP

anti- V5-tag antibody

6. Citations

  • Article Title: Anti-hyperuricemic effects of three theaflavins isolated from black tea in hyperuricemic mice
    IF: 3.197
    Journal: Journal of Functional Foods
    Cited Product: anti- GAPDH antibody
  • Article Title: lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer
    IF: 3.046
    Journal: OncoTargets and Therapy
    Cited Product: anti- GAPDH antibody
  • Article Title: miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
    IF: 2.482
    Journal: Biological Research
  • Article Title: Inhibition of NLRP3 and Golph3 ameliorates diabetes-induced neuroinflammation in vitro and in vivo
    IF: 5.955
    Journal: Aging
  • Article Title: Expression of gag-CA Gene of Jembrana Disease Virus with Cationic Liposomal and Chitosan Nanoparticle Delivery Systems as DNA Vaccine Candidates
    IF: 0
    Journal: Tropical Life Sciences Research
    Cited Product: anti- GFP tag antibody
  • Article Title: MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis
    IF: 6.543
    Journal: Oxidative Medicine and Cellular Longevity
    Cited Product: anti- MYC-tag antibody
  • Article Title: Natural variation in WHITE-CORE RATE 1 regulates redox homeostasis in rice endosperm to affect grain quality
    IF: 12.796
    Journal: The Plant Cell
  • Article Title: Differential Expression of Lonp1 Isoforms in Cancer Cells
    IF: 7.666
    Journal: Cells
    Cited Product: anti- GFP tag antibody