Products
MYC-tag antibody
- Product Name
- MYC-tag antibody
- Catalogue No.
- FNab05462
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- MYC-tag
- Alternative Names
- myc tag antibody
Application
- Tested Applications
- ELISA, WB, IP, IF
- Recommended dilution
- WB: 1:1000-1:8000; IP: 1:500-1:2000 ; IF: 1:10-1:100
Validated Images
Myc-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab05462(MYC tag antibody) at dilution of 1:4000
IP Result of anti-MYC-tag (IP: FNab05462, 4ug; Detection: FNab05462 1:1000) with Transfected HEK-293 cells lysate 300ug.
- Background
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.
