Products

GAPDH antibody

Synonyms:G3PD antibody, GAPD antibody, GAPDH antibody
Catalogue No.:FNab03343Reativity:Human, Mouse, Rat, Zebrafish
Host:MouseTested Application:ELISA, IHC, WB, IF, FC, IP
Clonality:monoclonalIsotype:IgG1
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Product Name
GAPDH antibody
Catalogue No.
FNab03343
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
4A2
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
glyceraldehyde-3-phosphate dehydrogenase
Alternative Names
G3PD antibody, GAPD antibody, GAPDH antibody
UniProt ID
P04406
Observed MW
36 kDa
Application
Tested Applications
ELISA, IHC, WB, IF, FC, IP
Recommended dilution
WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab03343(GAPDH antibody) at dilution of 1:10000
IP Result of anti-GAPDH (IP: FNab03343, 4ug; Detection: FNab03343 1:3000) with Hela cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab03343(GAPDH Antibody) at dilution of 1:100
Background
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.
IF: 5.6
Journal:
Frontiers in Pharmacology
Author:
Laboratorio de Neuropsicofarmacología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría "Ramón de la Fuente Mu?iz", Mexico City, Mexico
Cited Date:
2023-11-10
Product:
IF: 5.1
Journal:
Chemico-Biological Interactions
Author:
School of Medicine, Yichun University, 576 XueFu Road, Yuanzhou District, Yichun, 336000, PR China
Cited Date:
2024-03-08
Product:
IF: 4.06
Journal:
American Journal of Translational Research
Cited Date:
2022-10-14
Product:
IF: 3.2
Journal:
Journal of Molecular Histology
Author:
Catedrático CONACYT, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico
Cited Date:
2023-07-07
Product: