Products
GAPDH antibody
| Size | Price |
|---|---|
| 100µg | Inquiry |
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- GAPDH antibody
- Catalogue No.
- FNab03343
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 4A2
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Immunogen
- glyceraldehyde-3-phosphate dehydrogenase
- Alternative Names
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)|Peptidyl-cysteine S-nitrosylase GAPDH|GAPDH|GAPD antibody
- UniProt ID
- P04406
- Observed MW
- 36 kDa
- Tested Applications
- ELISA, IHC, WB, IF, FC, IP
- Recommended dilution
- WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200
HeLa cells were subjected to SDS PAGE followed by western blot with FNab03343(GAPDH antibody) at dilution of 1:10000
IP Result of anti-GAPDH (IP: FNab03343, 4ug; Detection: FNab03343 1:3000) with Hela cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab03343(GAPDH Antibody) at dilution of 1:100
- Background
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.
- Journal:
- Chemosphere
- Author:
- Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
- Cited Date:
- 2024-08-02
- Product:
- Journal:
- Frontiers in Pharmacology
- Author:
- Laboratorio de Neuropsicofarmacología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría "Ramón de la Fuente Mu?iz", Mexico City, Mexico
- Cited Date:
- 2023-11-10
- Product:
- Journal:
- Chemico-Biological Interactions
- Author:
- School of Medicine, Yichun University, 576 XueFu Road, Yuanzhou District, Yichun, 336000, PR China
- Cited Date:
- 2024-03-08
- Product:
- Journal:
- American Journal of Translational Research
- Cited Date:
- 2022-02-11
- Product:
- Journal:
- American Journal of Translational Research
- Cited Date:
- 2022-10-14
- Product:
- Journal:
- Frontiers in Molecular Biosciences
- Author:
- School of Public Health, Youjiang Medical University for Nationalities, Baise, China.
- Sample:
- BEAS-2B cells
- Cited Date:
- 2024-10-11
- Product:
- Journal:
- Journal of Molecular Histology
- Author:
- Catedrático CONACYT, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico
- Cited Date:
- 2023-07-07
- Product:
- Journal:
- Journal of Functional Foods
- Cited Date:
- 2020-03-20
- Product:
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).