Products
Flag tag antibody
- Product Name
- Flag tag antibody
- Catalogue No.
- FNab03154
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Flag tag
Application
- Tested Applications
- ELISA, WB, IF
- Recommended dilution
- WB: 1:1000-1:4000; IF: 1:10-1:100
Validated Images
Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03154(Flag tag antibody) at dilution of 1:1000
Immunofluorescent analysis of Transfected HEK-293 cells using FNab03154 (Flag tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is a rabbit polyclonal antibody raised against 3xFlag(3xDYKDDDDKT) sequence and recognizes the(3x)DYKDDDDK peptide and detects FLAG-tagged proteins.
