Products
MBP tag antibody
- Product Name
- MBP tag antibody
- Catalogue No.
- FNab05042
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 0D8
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- MBP-Tag
- Alternative Names
- Maltose Binding Protein antibody, MBP antibody, MBP Tag antibody
- Observed MW
- 40 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:1000-1:8000; IP: 1:5000-1:50000
Validated Images
Recombinant protein were subjected to SDS PAGE followed by western blot with FNab05042(MBP tag antibody) at dilution of 1:4000
IP Result of anti-MBP tag (IP: FNab05042, 5ug; Detection: FNab05042 1:20000) with Recombinant protein protein lysate 800ug.
- Background
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.
