Products
Tubulin-beta antibody
- Product Name
- Tubulin-beta antibody
- Catalogue No.
- FNab09105
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 8A10
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tubulin, beta 3
- Alternative Names
- Beta tubulin antibody, MC1R antibody, TUBB antibody, Tubulin-beta antibody
- UniProt ID
- Q13509
- Observed MW
- 50 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:10000-1:50000; IF: 1:20-1:200; IP: 1:500-1:2000; IHC: 1:50-1:500
Validated Images
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with FNab09105(Tubulin-beta Antibody) at dilution of 1:10000
IP Result of anti-Tubulin-beta (IP: FNab09105, 5ug; Detection: FNab09105 1:1000) with HeLa cells lysate 880ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab09105(Tubulin-beta Antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells using FNab09105 ( Tubulin-beta Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
