Products
GAPDH antibody
References
8
Category:
Research Area:
- SPECIFICATIONS
- CITATIONS
- Product Name
- GAPDH antibody
- Catalogue No.
- FNab03342
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- glyceraldehyde-3-phosphate dehydrogenase
- Alternative Names
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)|Peptidyl-cysteine S-nitrosylase GAPDH|GAPDH|GAPD antibody
- UniProt ID
- P04406
- Observed MW
- 36 kDa
Application
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:1000-1:5000; IHC: 1:100-1:500
Validated Images
various lysates were subjected to SDS PAGE followed by western blot with FNab03342(GAPDH antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab03342(GAPDH antibody) at dilution of 1:100
- Background
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation.
IF: 6.543
- Journal:
- Oxidative Medicine and Cellular Longevity
- Cited Date:
- 2021-09-10
- Product:
IF: 4.06
- Journal:
- American Journal of Translational Research
- Cited Date:
- 2021-07-30
- Product:
IF: 3.046
- Journal:
- OncoTargets and Therapy
- Cited Date:
- 2020-04-10
- Product:
IF: 1.785
- Journal:
- Experimental and Therapeutic Medicine
- Cited Date:
- 2020-06-26
- Product:
IF: 1.4
- Journal:
- Iranian Journal of Public Health
- Author:
- General Surgery Department, Nanjing Red Cross Hospital, Nanjing City, Jiangsu Province, 210000, China
- Cited Date:
- 2023-08-11
- Product:
IF: 0.496
- Journal:
- Translational Cancer Research
- Cited Date:
- 2022-09-23
- Product:
- Journal:
- DSpace Repository
- Cited Date:
- 2021-02-26
- Product:
- Journal:
- Research Square
- Cited Date:
- 2022-09-16
- Product:
