Products

GAPDH antibody

Synonyms:GAPD antibody, G3PD antibody, GAPDH antibody, OK/SW cl.12 antibody
Catalogue No.:FNab03342Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, IHC, WB
Clonality:polyclonalIsotype:IgG
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Product Name
GAPDH antibody
Catalogue No.
FNab03342
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
glyceraldehyde-3-phosphate dehydrogenase
Alternative Names
GAPD antibody, G3PD antibody, GAPDH antibody, OK/SW cl.12 antibody
UniProt ID
P04406
Observed MW
36 kDa
Application
Tested Applications
ELISA, IHC, WB
Recommended dilution
WB: 1:1000-1:5000; IHC: 1:100-1:500
Validated Images
various lysates were subjected to SDS PAGE followed by western blot with FNab03342(GAPDH antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab03342(GAPDH antibody) at dilution of 1:100
Background
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation.
IF: 1.4
Journal:
Iranian Journal of Public Health
Author:
General Surgery Department, Nanjing Red Cross Hospital, Nanjing City, Jiangsu Province, 210000, China
Cited Date:
2023-08-11
Product: