Products
GFP tag antibody
- Product Name
- GFP tag antibody
- Catalogue No.
- FNab03434
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 0D8
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- GFP tag
- Alternative Names
- CFP antibody, eGFP antibody, eYFP antibody, GFP antibody, GFP tag antibody, YFP antibody
- UniProt ID
- P42212
- Observed MW
- 30 kDa
Application
- Tested Applications
- ELISA, WB, IP, IF
- Recommended dilution
- WB: 1:2000-1:16000; IP: 1:1000-1:4000; IF: 1:20-1:200
Validated Images
eGFP-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03434(GFP antibody) at dilution of 1:10000
IP Result of anti-GFP tag (IP: FNab03434, 4ug; Detection: FNab03434 1:2000) with Transfected HEK-293 cells lysate 300ug.
- Background
- Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium(Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
