Products
Rat IL-6(Interleukin-6) ELISA Kit
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This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- ER0042
- Alias:
- Interleukin-6 ELISA Kit, IL-6 ELISA Kit, B-cell stimulatory factor 2 ELISA Kit, BSF-2 ELISA Kit, CTL differentiation factor ELISA Kit, CDF ELISA Kit, Hybridoma growth factor ELISA Kit, Interferon beta-2 ELISA Kit, IFN-beta-2 ELISA Kit, IL6 ELISA Kit, IFNB2 ELISA Kit
- Species:
- Rat
- Range:
- 62.5-4000pg/ml
- Sensitivity:
- 37.5pg/ml
| Size | Price |
|---|---|
| 96T | Inquiry |
| 48T | Inquiry |
Dispatch Time: About 3 working days
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- Rat IL-6(Interleukin-6) ELISA Kit
- Alias
- Interleukin-6 ELISA Kit, IL-6 ELISA Kit, B-cell stimulatory factor 2 ELISA Kit, BSF-2 ELISA Kit, CTL differentiation factor ELISA Kit, CDF ELISA Kit, Hybridoma growth factor ELISA Kit, Interferon beta-2 ELISA Kit, IFN-beta-2 ELISA Kit, IL6 ELISA Kit, IFNB2 ELISA Kit
- Catalogue No.
- ER0042
- Size
- 48T/96T
- Species
- Rat
- UniProt ID
- P20607
- Sample Type
- 血清，血浆，细胞培养上清液，细胞裂解液或组织裂解液，其它生物液体样品
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4小时
- Range
- 62.5-4000pg/ml
- Sensitivity
- 37.5pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with IL-6 , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
- Washing: Wash the plate twice without immersing.
- Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
- Washing: Wash the plate three times and immerse for 1min each time.
- Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.129 0.136 0.132 62.5 0.168 0.176 0.171 125 0.179 0.188 0.183 250 0.218 0.229 0.222 500 0.319 0.335 0.325 1000 0.61 0.641 0.622 2000 1.157 1.216 1.181 4000 2.011 2.114 2.052 
- Recovery
-
Add a certain amount of IL-6 into the sample. Calculate the recovery by comparing the measured value with the expected amount of IL-6 in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 87-105 98 EDTA plasma(n=10) 85-103 96 Heparin plasma(n=10) 94-105 100 - Linearity
-
Dilute the sample with a certain amount of IL-6 at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 86-103% 86-99% 86-98% EDTA plasma(n=10) 92-98% 90-101% 83-100% Heparin plasma(n=10) 81-100% 80-99% 86-98% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 121.8 501 1984 124.9 497.1 1995 Standard deviation 6.15 25.95 103.76 6.57 23.61 105.54 CV(%) 5.05 5.18 5.23 5.26 4.75 5.29 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
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- Rat Cat(Catalase) ELISA Kit
- Rat MPO(Myeloperoxidase) ELISA Kit
- Rat Casp3(Caspase 3) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
IF: 0.78
- Journal:
- Journal of Taibah University Medical Sciences
- Cited Date:
- 2019-07-23
- Product:
IF: 0.719
- Journal:
- European Journal of Inflammation
- Cited Date:
- 2018-05-14
- Product:
IF: 0.69
- Journal:
- Open Life Sciences
- Cited Date:
- 2021-02-26
- Product:
IF: 0.687
- Journal:
- Journal of Food and Nutrition Research
- Cited Date:
- 2018-12-19
- Product:
IF: 0.533
- Journal:
- Tropical Journal of Pharmaceutical Research
- Cited Date:
- 2021-10-08
- Product:
IF: 0.533
- Journal:
- Tropical Journal of Pharmaceutical Research
- Cited Date:
- 2022-06-17
- Product:
IF: 0.427
- Journal:
- Indian Journal of Animal Research
- Cited Date:
- 2022-09-02
- Product:
IF: 0.3
- Journal:
- Journal of Clinical Practice and Research
- Author:
- Department of Biophysics, Ayd?n Adnan Menderes University, Institute of Health Sciences, Ayd?n, Türkiye
- Sample:
- serum
- Cited Date:
- 2024-12-13
- Product:
-
- Rat INS(Insulin) ELISA Kit
- Rat MDA(Malonaldehyde) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat HbA1C(Glycosylated Hemoglobin/Hemoglobin A1c) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- Comparative Clinical Pathology
- Cited Date:
- 2019-06-25
- Product:
-
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat pτ(phospho Tau Protein) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat CASP9(Caspase 9) ELISA Kit
- Rat Aβ40(Amyloid Beta 40) ELISA Kit
- Rat BCL-2(B-Cell Leukemia/Lymphoma 2) ELISA Kit
- Rat Bax(Apoptosis regulator BAX) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
- Journal:
- Anesthesia and Pain Medicine
- Cited Date:
- 2020-06-30
- Product:
- Journal:
- Research in Pharmaceutical Sciences
- Cited Date:
- 2022-09-08
- Product:
- Journal:
- United States Patent Application
- Cited Date:
- 2020-07-16
- Product:
- Journal:
- Farabi Medical Journal
- Cited Date:
- 2023-01-29
- Product:
- Journal:
- Biointerface Research in Applied Chemistry
- Cited Date:
- 2023-02-09
- Product:
- Journal:
- Novosti Khirurgii
- Author:
- Department of Surgery and Transplantation, Belarusian State Medical University, Minsk, Dzerzhinsky Ave. 83, 220116, Republic of Belarus
- Cited Date:
- 2023-10-08
- Product:
- Journal:
- Environmental Analysis Health and Toxicology
- Author:
- Department of Human Anatomy, Kaduna State University, State, Nigeria
- Cited Date:
- 2024-01-19
- Product:
- Journal:
- Egyptian Journal of Chemistry
- Author:
- Pharmacognosy Department, National Research Centre (NRC), El Behouth St., P.O. 12622, Cairo, Egypt.
- Sample:
- serum
- Cited Date:
- 2024-11-22
- Product:
- Journal:
- Farabi Medical Journal
- Author:
- Karadeniz Technical University, Faculty of Health Sciences, Department of Nutrition and Dietetics, 61080 Trabzon, Türkiye
- Cited Date:
- 2023-06-09
- Product:
- Journal:
- Narra J
- Author:
- Department of Biochemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.
- Sample:
- serum
- Cited Date:
- 2025-02-21
- Product:
- Journal:
- International Journal of Multidisciplinary Research and Analysis
- Author:
- Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
- Sample:
- skin tissue
- Cited Date:
- 2024-05-06
- Product:
- Journal:
- Clinical and Experimental Health Sciences
- Author:
- KARADENIZ TECHNICAL UNIVERSITY
- Cited Date:
- 2023-10-20
- Product:
- Journal:
- Research Square
- Cited Date:
- 2022-05-05
- Product:
-
- Rat MDA(Malonaldehyde) ELISA Kit
- Rat TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat Gsr(Glutathione reductase) ELISA Kit
- Rat PTGS2/COX-2(Prostaglandin G/H synthase 2) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- AIP Conference Proceedings
- Cited Date:
- 2020-09-16
- Product:
- Journal:
- research square
- Cited Date:
- 2020-09-29
- Product:
-
- Rat PGE2(Prostaglandin E2) ELISA Kit
- Rat 8-iso-PGF2α(8-isoprostane) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat OVA sIgE(Ovalbumin specific Immunoglobulin E) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
- Rat IL-10(Interleukin-10) ELISA Kit
- Rat IL-13(Interleukin-13) ELISA Kit
- Rat IL-17(Interleukin-17) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Mouse Ltb4r(Leukotriene B4 receptor 1) ELISA Kit
- Journal:
- Journal of Advanced Veterinary and Animal Research
- Author:
- Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
- Sample:
- supernatant
- Cited Date:
- 2025-03-07
- Product:
- Journal:
- Research Square
- Author:
- Islamic Azad University
- Cited Date:
- 2024-02-18
- Product:
- Journal:
- Journal of Innovative Research in Life Sciences
- Author:
- Department of Biochemistry, Federal University Gusau, Zamfara State-Nigeria
- Sample:
- serum
- Cited Date:
- 2024-05-31
- Product:
- Journal:
- Recent Advances in Drug Delivery and Formulation
- Author:
- Department of Pharmacy, I.T.S. College of Pharmacy, Delhi-Meerut Road, Ghaziabad -201206, India.
- Cited Date:
- 2024-10-08
- Product:
- Journal:
- Farabi Medical Journal
- Cited Date:
- 2023-04-07
- Product:
- Journal:
- Medicinski Glasnik
- Cited Date:
- 2021-07-15
- Product:
- Journal:
- Sahel Journal of Life Sciences FUDMA
- Author:
- Department of Biochemistry and Molecular Biology, Federal University Dutsin-Ma, P.M.B 5001, Katsina State, Nigeria
- Sample:
- serum
- Cited Date:
- 2024-03-08
- Product:
- Journal:
- Journal of Nutrition and Health
- Cited Date:
- 2020-03-13
- Product:
- Journal:
- Comparative Clinical Pathology
- Cited Date:
- 2019-02-15
- Product:
-
- 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- MDA(Malondialdehyde) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Gpx1(Glutathione peroxidase 1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- F?rat University Medical Journal of Health Sciences
- Author:
- Gaziantep University, Faculty of Medicine, Department of Physiology, Gaziantep, T?RK?YE
- Cited Date:
- 2023-07-07
- Product:
- Journal:
- Phytomedicine Plus
- Author:
- Dairy Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
- Species:
- blood samples
- Cited Date:
- 2024-06-14
- Product:
- Journal:
- F1000Research
- Cited Date:
- 2022-12-16
- Product:
- Journal:
- bioRxiv
- Author:
- Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi, Nigeria
- Cited Date:
- 2023-09-15
- Product:
- Journal:
- Journal of International Dental and Medical Research
- Cited Date:
- 2020-05-02
- Product:
- Journal:
- Research Square
- Cited Date:
- 2022-03-10
- Product:
- Journal:
- HEPATITIS MONTHLY
- Cited Date:
- 2020-05-10
- Product:
-
- Rat MDA(Malonaldehyde) ELISA Kit
- Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat IL-1β(Interleukin 1 Beta) ELISA Kit
- Rat CASP9(Caspase 9) ELISA Kit
- Rat BCL-2(B-Cell Leukemia/Lymphoma 2) ELISA Kit
- Rat Bax(Apoptosis regulator BAX) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Gpx1(Glutathione peroxidase 1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
- Journal:
- Beni-Suef University Journal of Basic and Applied Sciences
- Cited Date:
- 2023-01-06
- Product:
-
- 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
- Rat ATF6(Activating transcription factor 6) ELISA Kit
- Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
- Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
- Rat Ddit3(DNA damage-inducible transcript 3 protein) ELISA Kit
- Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
- Rat SOD1(Superoxide dismutase [Cu-Zn]) ELISA Kit
- Rat Hmgb1(High mobility group protein B1) ELISA Kit
- Rat Cat(Catalase) ELISA Kit
- Rat MPO(Myeloperoxidase) ELISA Kit
- Rat Casp3(Caspase 3) ELISA Kit
- Rat IL-6(Interleukin-6) ELISA Kit
About FineTest® ELISA Kits
What’s the plate size in FineTest® ELISA Kits?
The ELISA plate follows the standard size of microplate: 127.64 mm x 85.60 mm x 14.22 mm(L x W x H).
How about the shelf life and stability of FineTest® ELISA Kits?
Valid for 12 months since the production date. For the shelf life of specific batch number, please check the label printed on the kit. Before delivery, all FineTest® ELISA Kits have been subject to strict quality test.
Which cloned antibodies for FineTest® ELISA Kits are used?
These information is proprietary. Please contact us to learn more about clonality (polyclonality or monoclonality) and host species.
Can I mix reagents from different batches of FineTest® ELISA Kits?
Not suggested. ELISA reagents are optimized for specific batch.
Can FineTest® ELISA Kits be used partially?
Yes. The ELISA plate is dismounted. Enough component volumes are offered by 96T ELISA kit, supporting two groups of standard curve.
How long can the diluted lyophilized standard be stored for continual use?
Used up within 12h.
Can standard curve be extended to any direction?
FineTest® can't support validation of standard concentration outside of standard curve. Ranges of standard curve have been validated among many batches and experimenters, showing stable and accurate performance. The lowest standard concentration is the minimized range for reliable detection results. Adding higher or lower concentration of standard may cause inconsistent signal or false positive.
Sample Issues
Why does detection for serum/plasma sample by FineTest® ELISA Kits require for 1/2 dilution?
Matrix components in serum/plasma can affect detection results. Blocking components in sample dilution buffer can decrease or remove the interference. The dilution can reduce the matrix difference between sample and standard to get better accuracy.
What’s the half-life of protein in serum/plasma/cell culture supernatant?
FineTest® can't determine the half-life of protein in the sample(e.g. serum, plasma or cell culture supernatant). Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container. Avoid freeze-thaw cycle to prevent protein degradation.
What's the expected concentration for particularly analyzing my sample?
Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics. Please contact us to get detection data for reference.
How many samples can FineTest® ELISA Kits detect?
It depends on whether duplicate assay for your sample is required. Such as, you can detect 80 samples without duplication or 40 samples.
Operational Issues
Why is sample loading conducted after equilibrating all kits’ components and samples to room temperature?
Temperature is the important factor for ELISA binding reaction. The reaction of samples at a consistent temperature requires for equilibrating all reagents to room temperature before the assay, including tested samples. Avoid the inaccurate ELISA assay results caused by temperature differences.
Why is duplicate assay suggested for ELISA assay?
To get more accurate assay results, it's strongly suggested to conduct duplicate assay for the standard and sample. Duplicate assay can calculate average value to ensure more accurate assay results and solve drift caused by misoperation during the assay. Calculation of CV evaluates experimental operation and precision of the ELISA kit.
How to get the standard curve with excellent linearity?
Follow the suggested method in the manual to store the standard. Before dissolving the standard, transient centrifugation is required to completely collect the powder. Confirm the fully dissolution and mixing of the standard(about 10min). Then, conduct steps of series dilution. Ensure the fully mixing and accurate pipetting in each step. Properly stop the staining. Choose suitable fitting equation to plot the standard curve.
Can ELISA plates be stacked together for incubation?
To keep the consistent environmental condition of all plates, stacking is not suggested during incubation.
Why is polypropylene tube used for standard dilution in some analyses?
Some proteins or analytes can bind with glass or polystyrene. However, they are not easy to bind with polypropylene tube.
When to stop ELISA reaction?
ELISA assay finally requires for enzyme-catalyzed substrate to complete the staining reaction. Stopping the reaction at the best time is the important factor for successful ELISA assay. When the blue complex appears after adding TMB substrate for 12min, read the O.D. absorbance at 620nm. The staining can be stopped, when the OD value of the darkest color at 620nm is between 0.8 - 0.9. The relevant OD value at 450nm for stopping the staining is between 2.0 - 2.5. If the OD value at 620nm is lower than 0.8, the staining duration can be properly extended.
Why is dual wavelength selected for reading in a microplate reader? What's the purpose of wavelength correction?
Dual wavelength for reading in ELISA assay mainly aims to remove non-specific background interference, and improve accuracy and reliability of the detection. FineTest® usually suggests to set the corrected wavelength as 570nm or 630nm in a microplate reader. The OD value may be higher when directly reading at 450nm. However, the accuracy is lower.
Why is 4 - pl curve fitting is required for generating standard curve?
FineTest® recommends to use ELISA curve fitting software and validate ELISA kit with 4 - pl curve fitting. 4 - pl curve fitting mainly aims to accurately describe the nonlinear dynamic characteristics of antigen - antibody binding. Thus, the quantitative accuracy of low or high concentration of sample is improved. Experimental errors are decreased. The reliability and reproducibility of assay results are ensured.
If sample OD value is higher than OD value of the highest point on the standard curve, what's the suggested solution?
Dilute the sample with dilution buffer and conduct the detection again. Ensure the detected value of the sample falls in the range of standard curve.
Publication Rewards
Are there any rewards to publish papers using FineTest® ELISA Kits?
If you published paper using FineTest® ELISA Kits, you will obtain US$150-US$550 coupon, please contact us for more details.
