Products

Phospho-Akt (Ser473) antibody

Synonyms:AKT antibody, AKT1 antibody, AKT1-phospho-S473 antibody, PKB antibody, PKB ALPHA antibody, PRKBA antibody, Protein kinase B antibody, Proto oncogene c Akt antibody, RAC antibody, RAC ALPHA antibody, RAC PK alpha antibody
Catalogue No.:FNab06402Reativity:Human, Mouse, Rat
Host:MouseTested Application:ELISA, IHC, WB
Clonality:monoclonalIsotype:IgG1
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Product Name
Phospho-Akt (Ser473) antibody
Catalogue No.
FNab06402
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
5H4
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
v-akt murine thymoma viral oncogene homolog 1
Alternative Names
AKT antibody, AKT1 antibody, AKT1-phospho-S473 antibody, PKB antibody, PKB ALPHA antibody, PRKBA antibody, Protein kinase B antibody, Proto oncogene c Akt antibody, RAC antibody, RAC ALPHA antibody, RAC PK alpha antibody
UniProt ID
P31749
Observed MW
60KD
Application
Tested Applications
ELISA, IHC, WB
Recommended dilution
WB: 1:1000-1:4000; IHC: 1:50-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06402(Phospho-Akt (S473) Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06402(Phospho-Akt (S473) Antibody) at dilution of 1:200
Background
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification.
IF: 5.2
Journal:
Cancers
Author:
Group of Experimental Biotherapy and Diagnostics, Institute for Regenerative Medicine, World-Class Research Centre “Digital Biodesign and Personalized Healthcare”, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia
Cited Date:
2023-11-10
Product: