Products
Phospho-Akt(Ser473) antibody
- Product Name
- Phospho-Akt(Ser473) antibody
- Catalogue No.
- FNab06402
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 5H4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- v-akt murine thymoma viral oncogene homolog 1
- Alternative Names
- RAC-alpha serine/threonine-protein kinase|Protein kinase B (PKB)|Protein kinase B alpha (PKB alpha)|Proto-oncogene c-Akt|RAC-PK-alpha|AKT1|PKB|RAC antibody
- UniProt ID
- P31749
- Observed MW
- 60 kDa
Application
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:1000-1:4000; IHC: 1:50-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab06402(Phospho-Akt (S473) Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06402(Phospho-Akt (S473) Antibody) at dilution of 1:200
- Background
- The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification.
