Products

ATG5 antibody

Synonyms:APG5L antibody, ASP antibody
Catalogue No.:FNab00678Reativity:Human, Mouse, Rat
Host:MouseTested Application:ELISA, WB
Clonality:monoclonalIsotype:IgG1
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Product Name
ATG5 antibody
Catalogue No.
FNab00678
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
7H7
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
ATG5 autophagy related 5 homolog
Alternative Names
APG5L antibody, ASP antibody
UniProt ID
Q9H1Y0
Observed MW
50-55 kDa
Application
Tested Applications
ELISA, WB
Recommended dilution
WB: 1:1000-1:3000
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab00678(ATG5 antibody) at dilution of 1:1000
Background
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
IF: 5.2
Journal:
Cancers
Author:
Group of Experimental Biotherapy and Diagnostics, Institute for Regenerative Medicine, World-Class Research Centre “Digital Biodesign and Personalized Healthcare”, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia
Cited Date:
2023-11-10
Product: