Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

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This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF-α antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TNF-α bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TNF-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
ER1393
Alias: 
Tumor necrosis factor ELISA Kit, Cachectin ELISA Kit, TNF-alpha ELISA Kit, Tumor necrosis factor ligand superfamily member 2 ELISA Kit, TNF-a ELISA Kit, Tumor necrosis factor, membrane form ELISA Kit, N-terminal fragment ELISA Kit, NTF ELISA Kit, Intracellular domain 1 ELISA Kit, ICD1 ELISA Kit, Intracellular domain 2 ELISA Kit, ICD2 ELISA Kit, C-domain 1 ELISA Kit, C-domain 2 ELISA Kit, Tumor necrosis factor, soluble form ELISA Kit, TNF ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Species: 
Rat
Range: 
3.906-250pg/ml
Sensitivity: 
2.344pg/ml
Manual MSDS
Size Price
96T Inquiry
48T Inquiry
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Product Name
Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Alias
Tumor necrosis factor ELISA Kit, Cachectin ELISA Kit, TNF-alpha ELISA Kit, Tumor necrosis factor ligand superfamily member 2 ELISA Kit, TNF-a ELISA Kit, Tumor necrosis factor, membrane form ELISA Kit, N-terminal fragment ELISA Kit, NTF ELISA Kit, Intracellular domain 1 ELISA Kit, ICD1 ELISA Kit, Intracellular domain 2 ELISA Kit, ICD2 ELISA Kit, C-domain 1 ELISA Kit, C-domain 2 ELISA Kit, Tumor necrosis factor, soluble form ELISA Kit, TNF ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
Catalogue No.
ER1393
Size
48T/96T
Species
Rat
UniProt ID
P16599
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
3.906-250pg/ml
Sensitivity
2.344pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with TNF-α , no obvious cross reaction with other analogues.
ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit
E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light)
E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul
E024 TMB Substrate 5ml 10ml
E039 Sample Dilution Buffer 10ml 20ml 2-8°C
E040 Antibody Dilution Buffer 5ml 10ml
E049 SABC Dilution Buffer 5ml 10ml
E026 Stop Solution 5ml 10ml
E038 Wash Buffer(Concentrated, 25X) 15ml 30ml
E006 Plate Sealer 3 pieces 5 pieces  
E007 Product Description 1 copy 1 copy
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Assay Procedure Summary
Elisa实验原理图
  • Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
  • Washing: Wash the plate twice without immersing.
  • Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
  • Washing: Wash the plate three times and immerse for 1min each time.
  • Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
  • Washing: Wash the plate five times and immerse for 1min each time.
  • Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
  • Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 0.088 0.092 0.09
3.906 0.111 0.116 0.113
7.813 0.134 0.14 0.137
15.625 0.264 0.274 0.269
31.25 0.433 0.45 0.442
62.5 0.742 0.772 0.757
125 1.242 1.292 1.267
250 2.005 2.087 2.046
ER1393 Standard Curve Image
Recovery

Add a certain amount of TNF-α into the sample. Calculate the recovery by comparing the measured value with the expected amount of TNF-α in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 88-96 93
EDTA plasma(n=10) 90-97 94
Heparin plasma(n=10) 86-104 96
Linearity

Dilute the sample with a certain amount of TNF-α at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 89-93% 86-105% 87-100%
EDTA plasma(n=10) 85-98% 82-96% 83-101%
Heparin plasma(n=10) 81-100% 81-99% 84-100%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 7.78 30.39 124.29 8.11 30.97 125.2
Standard deviation 0.48 1.34 5.56 0.36 1.46 6
CV(%) 6.2 4.42 4.47 4.5 4.72 4.79
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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IF: 2.4
Journal:
Medicina
Author:
Department of Physiology, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43020, Turkey
Sample:
supernatants
Cited Date:
2025-03-14
Product:
Efficiency of exogenous surfactant combined with intravenous N-acetylcysteine in two-hit rodent model of ARDS
IF: 2.3
Journal:
Respiratory Physiology & Neurobiology
Author:
Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala hora 4C, 036 01 Martin, Slovakia
Cited Date:
2023-08-18
Product:
Products of oxidative stress and transient receptor potential ankyrin A1 expression in the brainstem after lung ischemia–reperfusion injury
IF: 2.283
Journal:
Pulmonary Circulation
Cited Date:
2019-08-02
Product:
Improvement of lung ischemia-reperfusion injury by inhibition of microRNA-155 via reductions in neuroinflammation and oxidative stress of vagal afferent nerve
IF: 2.283
Journal:
Pulmonary Circulation
Cited Date:
2020-04-10
Product:
Products of oxidative stress and TRPA1 expression in the brainstem after lung ischemiareperfusion injury
IF: 2.178
Journal:
Pulmonary Circulation
Cited Date:
2019-06-25
Product:
A blockade of microRNA-155 signal pathway has a beneficial effect on neural injury after intracerebral haemorrhage via reduction in neuroinflammation and oxidative stress
IF: 2.11
Journal:
Archives of Physiology and Biochemistry
Cited Date:
2020-05-16
Product:
Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress
IF: 2.1
Journal:
European Journal of Trauma and Emergency Surgery
Author:
Department of Chemistry and Chemical Processing Technologies, Macka Vocational School, Karadeniz Technical University, 61750, Trabzon, Turkey
Cited Date:
2023-07-14
Product:
Vitex doniana Leaves Extract Ameliorates Alterations Associated with 7, 12-Dimethyl Benz[a]Anthracene-Induced Mammary Damage in Female Wistar Rats
IF: 2.029
Journal:
Nutrition and Cancer
Cited Date:
2020-03-28
Product:
Notoginsenoside R1 Suppresses Inflammatory Signaling and Rescues Renal Ischemia-Reperfusion Injury in Experimental Rats
IF: 1.98
Journal:
Medical Science Monitor
Cited Date:
2020-01-26
Product:
Resistant Dextrin Protects Rats Against Streptozotocin Induced Gestational Diabetes Mellitus via Alteration of TLR4/MyD88/NF-κB Signaling Pathway
IF: 1.9
Journal:
Alternative Therapies in Health and Medicine
Author:
School of Public Health, Xi’an Jiaotong University, Xi’an Shaanxi, China.
Sample:
blood samples
Cited Date:
2024-08-02
Product:
Flavonoid fraction from chayote (Sechium edule (Jacq.) Sw) leaves reduced malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) in hyperuricemic rats
IF: 1.797
Journal:
Nutrition & Food Science
Cited Date:
2021-09-29
Product:
Scopoletin Attenuates Intracerebral Hemorrhage-Induced Brain Injury and Improves Neurological Performance in Rats
IF: 1.685
Journal:
Neuroimmunomodulation
Cited Date:
2021-05-07
Product:
The protective role of corilagin on renal calcium oxalate crystal-induced oxidative stress, inflammatory response, and apoptosis via PPAR-γ and PI3K/Akt pathway in rats
IF: 1.638
Journal:
Biotechnology and Applied Biochemistry
Cited Date:
2020-10-30
Product:
β-Sitosterol Attenuates the Intracranial Aneurysm Growth by Suppressing TNF-α-Mediated Mechanism
IF: 1.615
Journal:
Pharmacology
Cited Date:
2019-08-30
Product:
Neural stem cell transplantation improves neurological function in focal cerebral ischemia through GDNF/PI3K/AKT axis
IF: 1.6
Journal:
Cellular and Molecular Biology
Author:
Department of Traumatic Surgery& Emergency Surgery, The 1st Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang,325000, China
Cited Date:
2023-10-13
Product:
Anti-Inflammatory Effects of Step Electrical Stimulation on Complete Freund’s Adjuvant (CFA) Induced Rheumatoid Arthritis Rats
IF: 1.354
Journal:
Journal of Nanoscience and Nanotechnology
Cited Date:
2019-04-20
Product:
Attenuation of mTOR Signaling Is the Major Response Element in the Rescue Pathway of Chronic Kidney Disease in Rats
IF: 1.351
Journal:
Neuroimmunomodulation
Cited Date:
2020-06-10
Product:
A topical formulation of Anacardium occidentale L. leaves extract enhances wound healing via mediating TNF-α and TGF-β
IF: 0.944
Journal:
Indian Journal of Experimental Biology
Author:
C U Shah College of Pharmacy, S.N.D.T. Women’s University, Juhu Campus, Juhu Rd, Santacruz (W), Mumbai - 400 049, Maharashtra, India
Cited Date:
2023-06-09
Product:
The Role of Pectin Hydrogel Systems Plus Iodine and Phyto Extract in Second-Degree Burn in Rats
IF: 0.9
Journal:
The Eurasian Journal of Medicine
Author:
Department of Pharmaceutical Technology and Management, Azerbaijan Medical University Faculty of Pharmacy Baku, Azerbaijan
Sample:
supernatant
Cited Date:
2024-11-01
Product:
Anti-inflammatory activity of Turkey source pumpkin seed oil in rat oedema model
IF: 0.877
Journal:
Acta Poloniae Pharmaceutica - Drug Research
Cited Date:
2020-03-13
Product:
Ethanolic extracts of babandotan leaves (Ageratum conyzoides L.) prevents inflammation and proteoglycan degradation by inhibiting TNF-α and MMP-9 on osteoarthritis rats induced by monosodium iodoacetate
IF: 0.841
Journal:
Asian Pacific Journal of Tropical Medicine
Cited Date:
2017-03-06
Product:
Gallic acid attenuates torsion/detorsion-induced testicular injury in rats through suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress
IF: 0.8
Journal:
Revista Internacional de Andrología
Author:
Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey
Cited Date:
2024-05-06
Product:
Tualang honey and DHA-rich fish oil reduce the production of pro-inflammatory cytokines in the rat brain following exposure to chronic stress
IF: 0.78
Journal:
Journal of Taibah University Medical Sciences
Cited Date:
2019-07-23
Product:
Hesperidin enhances angiogenesis via modulating expression of growth and inflammatory factor in diabetic foot ulcer in rats
IF: 0.719
Journal:
European Journal of Inflammation
Cited Date:
2018-05-14
Product:
Protocatechuic acid attenuates cerebral aneurysm formation and progression by inhibiting TNF-alpha/Nrf-2/NF-kB-mediated inflammatory mechanisms in experimental rats
IF: 0.69
Journal:
Open Life Sciences
Cited Date:
2021-02-26
Product:
High Fiber Diet Suppression of TLR4 and NFκβ Gene Expression Correlated with TNF-α and IL-6 Levels in Hypertriglyceridemia Rats
IF: 0.687
Journal:
Journal of Food and Nutrition Research
Cited Date:
2018-12-19
Product:
Protective effect of S-allyl cysteine against cerebral ischemia/reperfusion injury in experimental rats
IF: 0.533
Journal:
Tropical Journal of Pharmaceutical Research
Cited Date:
2021-10-08
Product:
Simvastatin suppresses cerebral aneurysm in rats through suppression of release of pro-inflammatory cytokines
IF: 0.533
Journal:
Tropical Journal of Pharmaceutical Research
Cited Date:
2022-06-17
Product:
Neuroprotective Effects of Ficus carica Seed Oil in Diabetic Neuropathy: A Preclinical Study in Wistar Rats.
IF: 0.3
Journal:
Journal of Clinical Practice and Research
Author:
Department of Biophysics, Ayd?n Adnan Menderes University, Institute of Health Sciences, Ayd?n, Türkiye
Sample:
serum
Cited Date:
2024-12-13
Product:
The effect of crocin on apoptotic, inflammatory, BDNF, Pt, and Aβ40 indicators and neuronal density of CA1, CA2, and CA3 regions of hippocampus in the model of Alzheimer suffering rats induced with trimethyltin chloride
Journal:
Comparative Clinical Pathology
Cited Date:
2019-06-25
Product:
Hepatocurative Effects of Alpha Lipoic Acid in 5-Fluorouracil-Induced Toxicity Involves Activation of TNF Alpha and NF-κβ Signaling Pathway
Journal:
Tropical Journal of Natural Product Research
Cited Date:
2021-02-26
Product:
Anti-Inflammatory Effect of Omega-3 Fish Oil Accelerates MyoD and Myogenin Expressions Following Rat Exercise-Induced Muscle Injury Model in a Dose-Dependent Manner
Journal:
Malaysian Journal of Medicine and Health Sciences (MJMHS)
Cited Date:
2020-06-30
Product:
Crataegus sinaica defatted methanolic extract ameliorated monosodium iodoacetate-induced oxidative stress andinhibited inflammation in a rat model of osteoarthritis
Journal:
Research in Pharmaceutical Sciences
Cited Date:
2022-09-08
Product:
Supplementation Impact of Spirulina platensis Ethanol Extract on Inflammatory Homeostasis Modulation of Rat Spleen at Different Ages
Journal:
HAYATI Journal of Biosciences
Author:
Benemerita Universidad Autonoma de Puebla, 3972, Centro de Agroecología, Instituto de Ciencias, Puebla, Puebla, Mexico.
Sample:
supernatant
Cited Date:
2025-02-14
Product:
MEDICINAL COMPOSITION OF AMARANTH ORIGIN FOR CARDIOVASCULAR TREATMENT
Journal:
United States Patent Application
Cited Date:
2020-07-16
Product:
Toxicological implications of sequential administration of herbal and conventional medicines: Evidence from an in vivo study on Azadirachta indica and artesunate in male Wistar rats
Journal:
Toxicology Research and Application
Cited Date:
2021-03-25
Product:
Improvement of Pharmaceutically Active Materials from the Fruit of the Prickly Pear (Opuntia ficus-indica) Using Ultrasound (UAE) and Microwave (MAE) Assisted Extractions
Journal:
Biointerface Research in Applied Chemistry
Cited Date:
2023-02-09
Product:
Application of Platelet-Rich Plasma in Severe Acute Experimental Pancreatitis
Journal:
Novosti Khirurgii
Author:
Department of Surgery and Transplantation, Belarusian State Medical University, Minsk, Dzerzhinsky Ave. 83, 220116, Republic of Belarus
Cited Date:
2023-10-08
Product:
Antagonistic effectiveness of Anacardium occidentale leaf extract on lead-acetate exposure-induced hepatorenal toxicity in rats
Journal:
Environmental Analysis Health and Toxicology
Author:
Department of Human Anatomy, Kaduna State University, State, Nigeria
Cited Date:
2024-01-19
Product:
Mesenchymal Stem Cell Transplantation Promotes Functional Recovery through MMP2/STAT3 Related Astrogliosis after Spinal Cord Injury
Journal:
International Journal of Stem Cells
Cited Date:
2019-07-30
Product:
Regulatory Effect of Secretome-Hypoxia Mesenchymal Stem Cells on TNF-α Level in Streptozotocin-induced Rats
Journal:
International Journal of Cell and Biomedical Science
Cited Date:
2023-02-10
Product:
Effects of porang glucomannan combined with a high-protein diet on oxidative stress, inflammation, and aging markers in D-galactose-induced rats
Journal:
Narra J
Author:
Department of Biochemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.
Sample:
serum
Cited Date:
2025-02-21
Product:
The Effect of Sacha Inchi Tempe on Blood Glucose, HOMA-IR, and TNF-ɑ in Rats with Metabolic Syndrome
Journal:
Indonesian Journal of Nutrition and Food
Author:
Department of Nutrition Sciences, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
Sample:
serum
Cited Date:
2025-02-28
Product:
The Effect of Sapodilla Leaf Extract (Manilkara Zapota LP Royen) on IL-1 and TNF-α Expression in Male White Rats Wistar Strain Induced with UVB Light
Journal:
International Journal of Multidisciplinary Research and Analysis
Author:
Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
Cited Date:
2024-05-06
Product:
N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats
Journal:
Clinical and Experimental Health Sciences
Author:
KARADENIZ TECHNICAL UNIVERSITY
Cited Date:
2023-10-20
Product:
The use of ultrasound (UAE) and microwaves (MAE) to improve the extraction of pharmaceutically active materials from the fruit of the prickly pear (Opuntia ficus-indica)
Journal:
Research Square
Cited Date:
2022-05-05
Product:
The effect of PRP in oxidative stress male rats induced by D-galactose
Journal:
AIP Conference Proceedings
Cited Date:
2020-09-16
Product:
Comparative Study of Nanosilymarin Loaded on Chitosan versus Free Silymarin in CCl4-Induced Hepatotoxicity in Female Rats
Journal:
Frontiers in Health Informatics
Author:
Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Basrah, Iraq
Sample:
serum
Cited Date:
2024-09-27
Product:
Protective Effect of S-Allyl Cysteine Against Neonatal Asthmatic Rats
Journal:
research square
Cited Date:
2020-09-29
Product:
Basil (Ocimum basilicum) Leaves Essential Oil Ameliorates GluR1 Receptor Expression, TNF-α Level, and Pain-like Behaviors in Post-operative Pain Setting
Journal:
Bali Journal of Anesthesiology
Cited Date:
2022-05-27
Product:
Chitosan/Alginate-loaded Astragalus hamosus shows ameliorating effects on lipid profile, inflammatory and hormonal parameters, and reduces miRNA-222 expression in Polycystic Ovary Syndrome Rats
Journal:
Research Square
Author:
Islamic Azad University
Cited Date:
2024-02-18
Product:
Downregulation of P2y14 Receptor Expression Ameliorates Diabetic Cardiac Autonomic Neuropathy by Inhibiting Glial Ferroptosis Via the Nrf2/Gpx4 Axis
Journal:
SSRN
Author:
School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Sample:
SCG and neuron culture medium
Cited Date:
2024-07-26
Product:
Effect of Omega-3 Fatty Acid on Osteoanabolic Markers and Bone-resorbing Cytokines in Ovariectomized Rats
Journal:
Journal of Innovative Research in Life Sciences
Author:
Department of Biochemistry, Federal University Gusau, Zamfara State-Nigeria
Sample:
serum
Cited Date:
2024-05-31
Product:
The Modulatory Role of L-carnitine on Inflammation in Experimentally-induced Testicular Torsion in Wistar Rats
Journal:
FUDMA Journal of Sciences
Author:
Department of Human Physiology, Faculty of Basic Medical Science, Ahmadu Bello University, Zaria, Nigeria.
Sample:
serum
Cited Date:
2024-08-02
Product:
Evaluating the effect of hydro-alcoholic extract of Phoenix dactylifera L. spathe on streptozotocin-induced diabetic rats
Journal:
Comparative Clinical Pathology
Cited Date:
2021-06-25
Product:
Antidiabetic, Antioxidant and Anti-Inflammatory Activities of Residual Aqueous Fraction of Ethulia conyzoides in Induced Type 2 Diabetic Rats
Journal:
Tropical Life Sciences Research
Cited Date:
2022-11-18
Product:
Assessment of the Therapeutic Potential of a Novel Phosphoramidate Acyclic Nucleosideon Induced Hepatocellular Carcinoma in Rat Model
Journal:
SSRN
Author:
Biotechnology Program, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology (E-JUST), 21934, New Borg El-Arab City, Alexandria, Egypt
Cited Date:
2024-03-01
Product:
Therapeutic Effect of Astaxanthin on 5-Fluorouracil-Induced Ovarian Damage in Rats
Journal:
Bilecik Seyh Edebali University Journal of Science
Author:
Department of Chemistry and Chemical Processing Technologies, Macka Vocational School, Karadeniz Technical University, 61750 Trabzon, Türkiye
Sample:
supernatants
Cited Date:
2024-06-07
Product:
Potential effects of incretin-based therapies on polycystic ovary syndrome in rats: a comparative study of linagliptin versus liraglutide
Journal:
International Journal of Research in Pharmaceutical Sciences
Cited Date:
2021-07-15
Product:
Synergistic effect of oral zinc and dietary M. oleifera Leaf Supplementation on Diabetes-induced Oxidative Stress and Inflammation
Journal:
Sahel Journal of Life Sciences FUDMA
Author:
Department of Biochemistry and Molecular Biology, Federal University Dutsin-Ma, P.M.B 5001, Katsina State, Nigeria
Sample:
serum
Cited Date:
2024-03-08
Product:
Phytosterols content of Keunnunjami germ and its antioxidative effects in adult rats
Journal:
Journal of Nutrition and Health
Cited Date:
2020-03-13
Product:
Immunomodulatory effect of synbiotic goat milk cheese on indomethacin-induced inflammation rats
Journal:
Journal of Advanced Veterinary Research
Author:
Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia.
Sample:
culture supernatants
Cited Date:
2025-01-10
Product:
Therapeutic potential of oxymatrine in impeding the cardiomyopathy in the STZ-nicotinamide induced diabetes through SIRT1/Nrf2 signaling activation
Journal:
Research Square
Cited Date:
2022-12-09
Product:
Gallic acid reduces inflammatory cytokines and markers of oxidative damage in a rat model of estradiol-induced polycystic ovary
Journal:
Comparative Clinical Pathology
Cited Date:
2019-02-15
Product:
Sinapic Acid Ameliorates Cisplatin-Induced Peripheral Neuropathy: An In Vivo and In Vitro Study
Journal:
F?rat University Medical Journal of Health Sciences
Author:
Gaziantep University, Faculty of Medicine, Department of Physiology, Gaziantep, T?RK?YE
Cited Date:
2023-07-07
Product:
Efficacy of nanoencapsulated Moringa oleifera L. seeds and Ocimum tenuiflorum L. leaves extracts incorporated in functional soft cheese on streptozotocin-induced diabetic rats
Journal:
Phytomedicine Plus
Author:
Dairy Department, Food Industries and Nutrition Research Institute, National Research Centre, Cairo 12622, Egypt
Species:
blood samples
Cited Date:
2024-06-14
Product:
Zinc Supplementation in Cytokine Regulation During LPS-induced Sepsis in Rodent
Journal:
Journal of International Dental and Medical Research
Cited Date:
2020-05-02
Product:
Selenium Attenuates Cholestasis-Induced Liver Injury and Fibrosis by Alleviating Liver Oxidative Stress and Inflammation in Rats
Journal:
Bulletin of Egyptian Society for Physiological Sciences
Cited Date:
2022-03-10
Product:
Effects of Hydroalcoholic Leaf Extract of Avicennia marina on Apoptotic, Inflammatory, Oxidative Stress, and Lipid Peroxidation Indices and Liver Histology of Type 1 Diabetic Rats
Journal:
HEPATITIS MONTHLY
Cited Date:
2020-05-10
Product:
Vanillic acid attenuates testicular ischemia/reperfusion injury in rats
Journal:
Beni-Suef University Journal of Basic and Applied Sciences
Cited Date:
2023-01-06
Product:
Rosuvastatin loaded bilosomes as an innovative therapeutic approach for radiation-induced cardiovascular alterations by targeting Nrf2/HO-1 pathway
Journal:
Egyptian Pharmaceutical Journal
Author:
Drug Radiation Research Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.
Sample:
heart homogenate
Cited Date:
2025-04-18
Product:
About FineTest® ELISA Kits
What’s the plate size in FineTest® ELISA Kits?

The ELISA plate follows the standard size of microplate: 127.64 mm x 85.60 mm x 14.22 mm(L x W x H).

How about the shelf life and stability of FineTest® ELISA Kits?

Valid for 12 months since the production date. For the shelf life of specific batch number, please check the label printed on the kit. Before delivery, all FineTest® ELISA Kits have been subject to strict quality test.

Which cloned antibodies for FineTest® ELISA Kits are used?

These information is proprietary. Please contact us to learn more about clonality (polyclonality or monoclonality) and host species.

Can I mix reagents from different batches of FineTest® ELISA Kits?

Not suggested. ELISA reagents are optimized for specific batch.

Can FineTest® ELISA Kits be used partially?

Yes. The ELISA plate is dismounted. Enough component volumes are offered by 96T ELISA kit, supporting two groups of standard curve.

How long can the diluted lyophilized standard be stored for continual use?

Used up within 12h.

Can standard curve be extended to any direction?

FineTest® can't support validation of standard concentration outside of standard curve. Ranges of standard curve have been validated among many batches and experimenters, showing stable and accurate performance. The lowest standard concentration is the minimized range for reliable detection results. Adding higher or lower concentration of standard may cause inconsistent signal or false positive.

Sample Issues
Why does detection for serum/plasma sample by FineTest® ELISA Kits require for 1/2 dilution?

Matrix components in serum/plasma can affect detection results. Blocking components in sample dilution buffer can decrease or remove the interference. The dilution can reduce the matrix difference between sample and standard to get better accuracy.

What’s the half-life of protein in serum/plasma/cell culture supernatant?

FineTest® can't determine the half-life of protein in the sample(e.g. serum, plasma or cell culture supernatant). Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container. Avoid freeze-thaw cycle to prevent protein degradation.

What's the expected concentration for particularly analyzing my sample?

Due to the specificity of each sample, it's hard to forecast and depend on sample preparation as well as analytical characteristics. Please contact us to get detection data for reference.

How many samples can FineTest® ELISA Kits detect?

It depends on whether duplicate assay for your sample is required. Such as, you can detect 80 samples without duplication or 40 samples.

Operational Issues
Why is sample loading conducted after equilibrating all kits’ components and samples to room temperature?

Temperature is the important factor for ELISA binding reaction. The reaction of samples at a consistent temperature requires for equilibrating all reagents to room temperature before the assay, including tested samples. Avoid the inaccurate ELISA assay results caused by temperature differences.

Why is duplicate assay suggested for ELISA assay?

To get more accurate assay results, it's strongly suggested to conduct duplicate assay for the standard and sample. Duplicate assay can calculate average value to ensure more accurate assay results and solve drift caused by misoperation during the assay. Calculation of CV evaluates experimental operation and precision of the ELISA kit.

How to get the standard curve with excellent linearity?

Follow the suggested method in the manual to store the standard. Before dissolving the standard, transient centrifugation is required to completely collect the powder. Confirm the fully dissolution and mixing of the standard(about 10min). Then, conduct steps of series dilution. Ensure the fully mixing and accurate pipetting in each step. Properly stop the staining. Choose suitable fitting equation to plot the standard curve.

Can ELISA plates be stacked together for incubation?

To keep the consistent environmental condition of all plates, stacking is not suggested during incubation.

Why is polypropylene tube used for standard dilution in some analyses?

Some proteins or analytes can bind with glass or polystyrene. However, they are not easy to bind with polypropylene tube.

When to stop ELISA reaction?

ELISA assay finally requires for enzyme-catalyzed substrate to complete the staining reaction. Stopping the reaction at the best time is the important factor for successful ELISA assay. When the blue complex appears after adding TMB substrate for 12min, read the O.D. absorbance at 620nm. The staining can be stopped, when the OD value of the darkest color at 620nm is between 0.8 - 0.9. The relevant OD value at 450nm for stopping the staining is between 2.0 - 2.5. If the OD value at 620nm is lower than 0.8, the staining duration can be properly extended.

Why is dual wavelength selected for reading in a microplate reader? What's the purpose of wavelength correction?

Dual wavelength for reading in ELISA assay mainly aims to remove non-specific background interference, and improve accuracy and reliability of the detection. FineTest® usually suggests to set the corrected wavelength as 570nm or 630nm in a microplate reader. The OD value may be higher when directly reading at 450nm. However, the accuracy is lower.

Why is 4 - pl curve fitting is required for generating standard curve?

FineTest® recommends to use ELISA curve fitting software and validate ELISA kit with 4 - pl curve fitting. 4 - pl curve fitting mainly aims to accurately describe the nonlinear dynamic characteristics of antigen - antibody binding. Thus, the quantitative accuracy of low or high concentration of sample is improved. Experimental errors are decreased. The reliability and reproducibility of assay results are ensured.

If sample OD value is higher than OD value of the highest point on the standard curve, what's the suggested solution?

Dilute the sample with dilution buffer and conduct the detection again. Ensure the detected value of the sample falls in the range of standard curve.

Publication Rewards
Are there any rewards to publish papers using FineTest® ELISA Kits?

If you published paper using FineTest® ELISA Kits, you will obtain US$150-US$550 coupon, please contact us for more details.

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