Products

Canine IL-6 (Interleukin 6) ELISA Kit

ECA0013 Catalogue No.
Reactivity
Canine
Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Product Name
Canine IL-6 (Interleukin 6) ELISA Kit
Catalogue No.
ECA0013
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Canine
UniProt ID
P41323
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9895
EDTA plasma(n=5)86-10193
heparin plasma(n=5)87-10399
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-103%96-103%88-104%89-103%
EDTA plasma(n=5)85-100%83-97%84-98%87-94%
heparin plasma(n=5)83-97%80-97%80-95%80-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only