- Product Name
- Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in plasma, tissue homogenates and other biological fluids.
- < 9.375pg/ml
- UniProt ID
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-102 94 EDTA plasma(n=5) 86-105 98 heparin plasma(n=5) 87-105 98
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-104% 90-105% 85-105% 90-105% EDTA plasma(n=5) 82-100% 83-95% 82-99% 87-101% heparin plasma(n=5) 83-94% 81-91% 84-90% 82-100%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area