Products

Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

ECA0020 Catalogue No.
Reactivity
Canine
Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Product Name
Canine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
Catalogue No.
ECA0020
Detection Method
Sandwich ELISA, Double Antibody
Application
TNF-α ELISA Kit allows for the in vitro quantitative determination of TNF-α concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Canine
UniProt ID
P51742
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of TNF-α and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10294
EDTA plasma(n=5)86-10598
heparin plasma(n=5)87-10598
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-104%90-105%85-105%90-105%
EDTA plasma(n=5)82-100%83-95%82-99%87-101%
heparin plasma(n=5)83-94%81-91%84-90%82-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only