Products

Canine IL-10 (Interleukin 10) ELISA Kit

ECA0012 Catalogue No.
Reactivity
Canine
Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Product Name
Canine IL-10 (Interleukin 10) ELISA Kit
Catalogue No.
ECA0012
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Canine
UniProt ID
P48411
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10196
EDTA plasma(n=5)90-10395
heparin plasma(n=5)87-10497
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)89-102%86-102%89-103%92-105%
EDTA plasma(n=5)82-101%85-93%84-97%87-98%
heparin plasma(n=5)82-99%81-100%83-99%86-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only