Abstract: Ulcerative colitis(UC) is a kind of chronic non-specific inflammatory bowel disease, involved in colorectum. The pathogenesis is related to genetic susceptibility, abnormal activation of immunity, intestinal microflora imbalance and environmental effects(e.g. diet, smoking). In recent years, conversion of anti inflammatory for ulcerative colitis to precision-targeted therapy include new biological reagents(e.g. IL-23, TL1A inhibitors) and …

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Abstract: Roles of accurate antibody selection in flow cytometry is very important for murine immunological research. Researchers are always puzzled at selection of specific immune cell markers, e.g. CD45.1/CD45.2, CD90.1/CD90.2 etc. Differences of these tissue-specific markers below provide selection guidelines. These tips help you precisely design flow cytometry assay. It’s easier and more efficient to …

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Abstract: Cardiac fibrosis is the key pathological feature of heart failure and various cardiomyopathy. Symptoms include excessive deposition of myocardial extracellular matrix induced myocardial stiffness and systolic and diastolic dysfunction. Key mechanism is differentiation of damage stimulated cardiac fibroblasts to activated myofibroblast(MyoFBs). MyoFBs highly expresses ACTA2, and massively secretes ECM proteins via transcriptional reprogramming. In …

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Abstract: During immunological research of infectious diseases, scarcity of clinical samples always restricts to obtain complete immune information. In the journal Cytometry Part A, a OMIP-114 project introduces 36-color spectral flow cytometry. This project only requires 200μL whole blood to conduct deep immunophenotype analysis for lymphocyte subsets and dysfunctional T cells(e.g. T/B/NK cell). This technology …

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Abstract: IL-25(also called IL-17E) is the member of IL-17 cytokine family, secreted by activated Th2 cell, mast cell, eosinophils and epithelial cells of respiratory/gastrointestinal tract and skin. Molecular structure includes typical cystine knot. IL-25 takes effects via binding with heterodimeric receptor(IL-17RA/IL-17RB complex). IL-17RB is the specific recognition subunit. Its expression in Th2 cell, eosinophils, mast …

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Abstract: Pathogens across body barrier will be subject to innate immune defense of complement system. Complement is the soluble protein in body fluid, and named for its contribution to antibody sterilization. Activation of antibody-dependent / independent pathway promotes clearance and phagocytosis of pathogens. Defensive roles in early infection are important. Besides anti-infection, complement is also …

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Abstract: Hidradenitis suppurativa(HS) is the chronic inflammatory skin disease. Symptoms include recurrent abscess around hair follicles, sinus tracts and scars. Currently, treatments usually focuses on patients in the Hurley II-III phase instead of Hurley I. Mild patients are still accompanied by severe pain, restricted function and psychological burden. Damage of persistent skin lesions may be …

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Abstract: Cell sorting is an advanced cell analysis method utilizing flow cytometry sorter. This technology detects intensity of scattered light and emitted fluorescence following excitation of high-energy laser to fluorescence labeled single cell or particle. These optical signals enable rapid and accurate qualitative and quantitative analysis for cell morphology, physiological and biochemical characteristics, immunophenotype, genetic …

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