Products
CASP3 antibody
- Product Name
- CASP3 antibody
- Catalogue No.
- FNab01289
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- caspase 3, apoptosis-related cysteine peptidase
- Alternative Names
- Caspase-3 (CASP-3)|Apopain|Cysteine protease CPP32 (CPP-32)|Protein Yama|SREBP cleavage activity 1 (SCA-1)|Caspase-3 subunit p17|Caspase-3 subunit p12|CASP3|CPP32 antibody
- UniProt ID
- P42574
- Observed MW
- 32-35 kDa
Application
- Tested Applications
- ELISA, IHC, WB,IF,IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab01289(CASP3 antibody) at dilution of 1:1000
IP result of anti-Caspase 3(IP:FNab01289, 4ug; Detection:FNab01289 1:300) with NIH/3T3 cells lysate 3440 ug
Immunohistochemistry of paraffin-embedded human spleen using FNab01289(CASP3 antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed NIH/3T3 cells using FNab01289 (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.
