Products

p65/RELA antibody

Synonyms:NFKB 3 antibody, NFKB p65 antibody, NFKB3 antibody, p65 antibody, RELA antibody, Transcription factor p65 antibody
Catalogue No.:FNab09875Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, IHC, IF, WB
Clonality:polyclonalIsotype:IgG
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Product Name
p65/RELA antibody
Catalogue No.
FNab09875
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
v-rel reticuloendotheliosis viral oncogene homolog A(avian)
Alternative Names
NFKB 3 antibody, NFKB p65 antibody, NFKB3 antibody, p65 antibody, RELA antibody, Transcription factor p65 antibody
UniProt ID
Q04206
Observed MW
65 kDa
Application
Tested Applications
ELISA, IHC, IF, WB
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09875(p65 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded rat lung using FNab09875(p65 antibody) at dilution of 1:50
Background
Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.