Products

anti- p65/RELA antibody

FNab09875 Catalogue No.
Synonyms
NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, IHC, IF, WB
Product Information
Product Name
anti- p65/RELA antibody
Catalogue No.
FNab09875
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Nuclear factor k B(NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105(NFkB1), p65(RelA), p52/p100(NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Immunogen Information
Immunogen
v-rel reticuloendotheliosis viral oncogene homolog A(avian)
Synonyms
NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65
Observed MW
65 kDa
Application
Tested Application
ELISA, IHC, IF, WB
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Uniprot information
UniProt ID
Q04206
Images
Immunohistochemistry of paraffin-embedded rat lung using FNab09875(p65 antibody) at dilution of 1:50
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09875(p65 antibody) at dilution of 1:500