WB: 1:500-1:3000; IF : 1:10-1:100; IHC: 1:50 - 1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab02404( DLAT Antibody) at dilution of 1:2000
IP Result of anti-DLAT (IP:FNab02404, 3ug; Detection:FNab02404 1:1000) with HepG2 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human kidney using FNab02404( DLAT Antibody) at dilution of 1:100
Immunofluorescent analysis of Hela cells using FNab02404 (DLAT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.