Products
c-MYC antibody
- Product Name
- c-MYC antibody
- Catalogue No.
- FNab01791
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- v-myc myelocytomatosis viral oncogene homolog(avian)
- Alternative Names
- BHLHe39 antibody, c Myc antibody, c-Myc antibody, MRTL antibody, MYC antibody, Myc proto oncogene protein antibody, Proto oncogene c Myc antibody, Transcription factor p64 antibody
- UniProt ID
- P01106
- Observed MW
- 62-65 kDa, 50 kDa
Application
- Tested Applications
- ELISA, WB, IP, IHC
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:50-1:200
Validated Images
Raji cells were subjected to SDS PAGE followed by western blot with FNab01791(MYC antibody) at dilution of 1:1000
IP Result of anti-MYC (IP: FNab01791, 3ug; Detection: FNab01791 1:500) with HL-60 cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab01791(MYC antibody) at dilution of 1:50
- Background
- MYC contains one basic helix-loop-helix(bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186]
