Products
P21;CDKN1A antibody
- Product Name
- P21;CDKN1A antibody
- Catalogue No.
- FNab06067
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- cyclin-dependent kinase inhibitor 1A(p21, Cip1)
- Alternative Names
- CAP20 antibody, CDK interacting protein 1 antibody, CDKN1 antibody, CDKN1A antibody, CIP1 antibody, MDA 6 antibody, MDA6 antibody, P21 antibody, p21CIP1 antibody, PIC1 antibody, SDI1 antibody, WAF1 antibody
- UniProt ID
- P38936
- Observed MW
- 21 kDa
Application
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06067(P21 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human kidney using FNab06067(P21 antibody) at dilution of 1:50
- Background
- CDKN1A(p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or-CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen(PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
