Products
NFE2L2 antibody
| Synonyms： | Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2 antibody, NFE2-related factor 2 antibody, Nrf-2)|HEBP1|Nuclear factor antibody, erythroid derived 2 antibody, like 2|NFE2L2|NRF2 antibody | ||
| Catalogue No.： | FNab05855 | Reactivity： | Human, Mouse, Rat, Zebrafish |
| Host： | Rabbit | Tested Application： | ELISA, IP, WB, IHC, FC |
| Clonality： | polyclonal | Isotype： | IgG |
| Size | Price |
|---|---|
| 100µg | Inquiry |
- SPECIFICATIONS
- CITATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- NFE2L2 antibody
- Catalogue No.
- FNab05855
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Immunogen
- nuclear factor(erythroid-derived 2)-like 2
- Alternative Names
- Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2 antibody, NFE2-related factor 2 antibody, Nrf-2)|HEBP1|Nuclear factor antibody, erythroid derived 2 antibody, like 2|NFE2L2|NRF2 antibody
- UniProt ID
- Q16236
- Observed MW
- 70-100 kDa
- Tested Applications
- ELISA, IP, WB, IHC, FC
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500
DMSO treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab05855(NFE2L2 Antibody) at dilution of 1:600
IP Result of anti-NFE2L2 (IP: FNab05855, 3ug; Detection: FNab05855 1:300) with mouse kidney tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using FNab05855(NFE2L2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response(ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress(PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC.
- Journal:
- Antioxidants
- Cited Date:
- 2021-07-23
- Product:
- Journal:
- Chemico-Biological Interactions
- Author:
- School of Medicine, Yichun University, 576 XueFu Road, Yuanzhou District, Yichun, 336000, PR China
- Cited Date:
- 2024-03-08
- Product:
- Journal:
- Life Sciences
- Cited Date:
- 2022-05-27
- Product:
- Journal:
- Toxicology Letters
- Author:
- School of Medicine, Yichun University,576 XueFu Road, Yuanzhou District,Yichun 336000,PR China.
- Cited Date:
- 2024-05-17
- Product:
- Journal:
- Journal of Biochemical and Molecular Toxicology
- Author:
- Department of Gastroenterology, Ministry of Health Eskisehir City Hospital, Eskisehir, Turkey.
- Cited Date:
- 2025-02-28
- Product:
- Journal:
- Journal of Biochemical and Molecular Toxicology
- Author:
- Department of Gastroenterology, Ministry of Health Eskisehir City Hospital, Eskisehir, Turkey.
- Cited Date:
- 2025-01-17
- Product:
- Journal:
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Author:
- Department of Gastroenterology, Ministry of Health Eskisehir City Hospital, 26080, Eskisehir, Turkey.
- Cited Date:
- 2025-02-28
- Product:
- Journal:
- Tissue and Cell
- Cited Date:
- 2022-08-05
- Product:
- Journal:
- SSRN
- Cited Date:
- 2022-02-11
- Product:
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).