Products
ERK1/2 antibody
- Product Name
- ERK1/2 antibody
- Catalogue No.
- FNab02846
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mitogen-activated protein kinase 1
- Alternative Names
- ERK antibody, ERK 2 antibody, ERK1/2 antibody, ERK2 antibody, ERT1 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK1 antibody, MAPK2 antibody, p40 antibody, p41 antibody, p41mapk antibody, p42 MAPK antibody, P42MAPK antibody, PRKM1 antibody, PRKM2 antibody
- UniProt ID
- P28482
- Observed MW
- 38-44 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:3000; IHC: 1:50-1:500; IF: 1:10-1:100
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02846(ERK1/2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab02846(ERK1/2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of Hela cells, using FNab02846(ERK1/2 Antibody) at 1:25 dilution. Blue pseudocolor = DAPI (fluorescent DNA dye).
- Background
- ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
