Products

Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit

EH0551 Catalogue No.
HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Reactivity
Human
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit
Catalogue No.
EH0551
Alias
HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HIF1A ELISA Kit allows for the in vitro quantitative determination of HIF1A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Q16665
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of HIF1A and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1A in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-10598
EDTA plasma(n=5)87-9491
heparin plasma(n=5)88-10396
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)91-104%85-98%85-105%
EDTA plasma(n=5)87-101%84-92%82-100%
heparin plasma(n=5)80-96%85-93%82-88%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only