Products

anti- LC3 antibody

FNab04717 Catalogue No.
Synonyms
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC, IF, IP
Product Information
Product Name
anti- LC3 antibody
Catalogue No.
FNab04717
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.1% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18-kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16-kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
Immunogen Information
Immunogen
microtubule-associated protein 1 light chain 3 beta
Synonyms
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B
Observed MW
16 kDa
Application
Tested Application
ELISA, WB, IHC, IF, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200; IF: 1:20-1:200
Uniprot information
UniProt ID
Q9GZQ8
Images
Immunohistochemistry of paraffin-embedded human gliomas using FNab04717 (LC3 antibody) at dilution of 1:50
IP Result of anti-LC3 (IP: FNab04717, 3ug; Detection: FNab04717 1:1000) with mouse brain tissue lysate 4000ug.
Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab04717 (LC3 Antibody) at dilution of 1:1000